Dale G L, Daniels R B
Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, CA.
Blood. 1991 Mar 1;77(5):1096-9.
The biochemical processes that determine the lifespan of mammalian erythrocytes are unknown; one prominent theory suggests that antibody binding to the senescent red blood cell identifies it for removal from the circulation. To address this question, we have used a newly developed procedure for the isolation of aged erythrocytes that involves the biotinylation of rabbit red blood cells, in vivo aging of these cells, and the eventual in vitro recovery of the aged red blood cells by their affinity for an avidin support. Erythrocytes isolated with this method were found to have near-normal levels of cell-associated Ig throughout their 60-day lifespan. These data suggest that IgG accumulation is not part of the normal senescence process for erythrocytes in rabbits.
决定哺乳动物红细胞寿命的生化过程尚不清楚；一个著名的理论认为，抗体与衰老红细胞的结合会识别该细胞以便将其从循环中清除。为了解决这个问题，我们采用了一种新开发的分离衰老红细胞的方法，该方法包括对兔红细胞进行生物素化、使这些细胞在体内老化，以及最终通过衰老红细胞对抗生物素蛋白载体的亲和力在体外回收它们。用这种方法分离的红细胞在其60天的寿命中，细胞相关免疫球蛋白水平接近正常。这些数据表明，IgG积累不是兔红细胞正常衰老过程的一部分。
