Suzuki T, Dale G L
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1647-51. doi: 10.1073/pnas.85.5.1647.
Rabbit erythrocytes were covalently labeled with biotin and then infused into the donor animal. Up to 60 days after infusion, the biotinylated cells were selectively isolated by their affinity for avidin. These aged erythrocytes, which are within 10 days of death, were analyzed for several biochemical parameters. The 2,3-bisphosphoglycerate and glutathione levels of these cells were constant with age; however, the adenosine 5'-triphosphate concentrations increased approximately 75% as the cells approached the end of their life span. The activities of several glycolytic enzymes did not change with age.
兔红细胞用生物素进行共价标记，然后注入供体动物体内。注入后长达60天，通过生物素化细胞对抗生物素蛋白的亲和力将其选择性分离。对这些距死亡10天内的衰老红细胞进行了几种生化参数分析。这些细胞的2,3-二磷酸甘油酸和谷胱甘肽水平随年龄保持恒定；然而，随着细胞接近其寿命末期，三磷酸腺苷浓度增加了约75%。几种糖酵解酶的活性并未随年龄变化。
