Institute of Otolaryngology, Catholic University, Rome, Italy.
Int J Audiol. 2009 Nov;48(11):804-10. doi: 10.3109/14992020903023140.
This study analysed the acoustic and vestibular functional and morphological modifications in guinea pigs after acoustic trauma. Animals were exposed to noise (6 kHz, at 120 dB SPL for 60 minutes) and then auditory brainstem responses (ABR) and vestibuloocular reflex (VOR) were measured at 6 hours, 1 day, 3, 7, and 21 days after noise. Western blotting and immunostaining for 4-hydroxy-2-noneal (4-HNE) and vascular endothelial growth factor (VEGF) were performed in the cochlear and vestibular regions at 1 and 7 days after noise exposure. A significant decrease of VOR gain was observed on day 1 and the recovery was completed at day 21. ABR threshold values reached a level of 80 dB at day 1 after trauma reaching a value of about 50 dB SPL on day 21. 4-HNE expression, a marker of lipid peroxidation was strongly increased in the cochlea. In the vestibule, 4-HNE immunoreactivity was faint. However, VEGF was up-regulated both in the cochlea and vestibule. In conclusion, the expression of VEGF in both cochlear and vestibular structures suggests a reparative role with potentially therapeutic implications.
本研究分析了豚鼠在噪声损伤后的听觉和前庭功能及形态学变化。动物暴露于噪声（6 kHz，120 dB SPL 持续 60 分钟），然后在噪声暴露后 6 小时、1 天、3 天、7 天和 21 天测量听性脑干反应（ABR）和前庭眼反射（VOR）。在噪声暴露后 1 天和 7 天，在耳蜗和前庭区域进行 4-羟基-2-壬烯醛（4-HNE）和血管内皮生长因子（VEGF）的 Western 印迹和免疫染色。VOR 增益在第 1 天显著下降，第 21 天恢复完成。ABR 阈值在创伤后第 1 天达到 80 dB，第 21 天达到约 50 dB SPL。耳蜗中脂质过氧化的标志物 4-HNE 表达强烈增加。在前庭中，4-HNE 免疫反应性较弱。然而，VEGF 在耳蜗和前庭结构中均上调。总之，VEGF 在耳蜗和前庭结构中的表达表明其具有修复作用，可能具有治疗意义。
