Xing Yan, Song Hong-mei, Li Tai-sheng, Qiu Zhi-feng, Wu Xiao-yan, Wang Wei, Wei Min
Department of Pediatrics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Er Ke Za Zhi. 2009 Jun;47(6):441-5.
To study the characteristics of the peripheral blood lymphocyte subsets in pediatric patients with chronic active EBV (CAEBV) infection.
Flow cytometry was used to detect the peripheral blood NK, B, T lymphocyte subsets and the functional, regulatory, naïve, memory and activatory subsets of T lymphocytes in 10 pediatric patients with CAEBV infection, 13 pediatric patients with acute Epstein-Barr virus infection (AEBV) and 12 healthy children in our hospital between March 2004 and April 2008.
Compared with AEBV group, the number of white blood cells [3325 x 10(6)/L (median, just the same as the following)], lymphocytes (1078 x 10(6)/L), NK cells (68 x 10(6)/L), B cells (84 x 10(6)/L), total T cells (684 x 10(6)/L), CD4+ T cells (406 x 10(6)/L) and CD8+ T cells (295 x 10(6)/L) in CAEBV patients were lower (P<0.05). The functional subset of the CD4+ T cells in CAEBV group (94.5%) was lower than those of the healthy control group (98.7%) (P<0.05), but was still higher than those of AEBV group (74.0%) (P<0.05). While the functional subset of the CD8+ T cells in CAEBV (40.7%) was not dramatically different from the healthy control group (48.3%), but was still higher than that of AEBV group (21.0%) (P<0.05). Although the regulatory subset in CAEBV group (5.0%) was higher than the health control group (4.6%) (P<0.05), but lower than AEBV group (5.8%) (P<0.05). In CAEBV, the proportion of CD4+/CD8+ naïve T cells (32.3%/37.5%) was lower than that of normal group (58.3%/56.6%) (P<0.05), but the proportion of CD4+/CD8+ effective memory T cells in CAEBV group (23.9%/15.1%) was lower than that in AEBV group (36.5%/69.8%) (P<0.05), while the proportion of CD8+ fake naïve T cells in CAEBV (17.5%) was higher than the other 2 groups (P<0.05). The CD8+ activatory subset in CAEBV group (84.4%/34.0%) was higher than that of the healthy control group (44.1%/16.7%) (P<0.05), but still lower than AEBV group (96%/95%) (P<0.05).
There is an imbalance in lymphocyte subsets and disturbance in cellular immunity in CAEBV patients, which may be associated with EBV chronic active infection. Detecting the peripheral haematologic parameters and lymphocyte subsets may be helpful in the diagnosis and the differential diagnosis of CAEBV.
研究慢性活动性EB病毒（CAEBV）感染患儿外周血淋巴细胞亚群的特征。
采用流式细胞术检测2004年3月至2008年4月我院10例CAEBV感染患儿、13例急性EB病毒感染（AEBV）患儿及12例健康儿童外周血NK、B、T淋巴细胞亚群以及T淋巴细胞的功能、调节、初始、记忆和活化亚群。
与AEBV组相比，CAEBV组患儿白细胞[3325×10⁶/L（中位数，下同）]、淋巴细胞（1078×10⁶/L）、NK细胞（68×10⁶/L）、B细胞（84×10⁶/L）、总T细胞（684×10⁶/L）、CD4⁺T细胞（406×10⁶/L）及CD8⁺T细胞（295×10⁶/L）数量均较低（P<0.05）。CAEBV组CD4⁺T细胞功能亚群（94.5%）低于健康对照组（98.7%）（P<0.05），但高于AEBV组（74.0%）（P<0.05）。CAEBV组CD8⁺T细胞功能亚群（40.7%）与健康对照组（48.3%）差异无统计学意义，但高于AEBV组（21.0%）（P<0.05）。CAEBV组调节亚群（5.0%）高于健康对照组（4.6%）（P<0.05），但低于AEBV组（5.8%）（P<0.05）。CAEBV组CD4⁺/CD8⁺初始T细胞比例（32.3%/37.5%）低于正常组（58.3%/56.6%）（P<0.05），CAEBV组CD4⁺/CD8⁺有效记忆T细胞比例（23.9%/15.1%）低于AEBV组（36.5%/69.8%）（P<0.05），而CAEBV组CD8⁺假初始T细胞比例（17.5%）高于其他两组（P<0.05）。CAEBV组CD8⁺活化亚群（84.4%/34.0%）高于健康对照组（44.1%/16.7%）（P<0.05），但低于AEBV组（96%/95%）（P<0.05）。
CAEBV患儿存在淋巴细胞亚群失衡及细胞免疫紊乱，可能与EB病毒慢性活动性感染有关。检测外周血液学参数及淋巴细胞亚群可能有助于CAEBV的诊断及鉴别诊断。
