State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, 17 Changle Western Road, Xi'an, China.
Gut. 2010 May;59(5):579-85. doi: 10.1136/gut.2008.175497. Epub 2009 Nov 30.
Several microarray studies have reported microRNA (miRNA) expression signatures that classify cancer patients into different prognostic groups. No study has evaluated the association between miRNA expression patterns and gastric cancer prognosis. In this study, we developed a seven-miRNA signature that is closely associated with survival of patients with gastric cancer.
MiRNA expression profile was analysed by real-time RT-PCR in 100 gastric cancer patients, which were randomly assigned to either the training set or the testing set. Cox proportional hazard regression and risk-score analysis were used to identify a stage-independent set of seven-miRNA signature in the training set that could classify patients with significantly different prognosis. This miRNA signature was further validated by the testing set and an independent cohort 60 patients.
We have identified a seven-miRNA signature (miR-10b, miR-21, miR-223, miR-338, let-7a, miR-30a-5p, miR-126) for overall survival (p=0.0009) and relapse-free survival (p=0.0005) of gastric cancer patients. Multivariate analysis shown that the risk signature was an independent predictor of overall survival (HR=3.046; 95% CI, 1.246 to 7.445, p=0.015) and relapse-free survival (HR=3.337; 95% CI, 1.298 to 8.580, p=0.012). Furthermore, the predictive value of this seven-miRNA signature was validated in the testing set of 50 patients and an independent set of 60 patients.
Our seven-miRNA signature is closely associated with relapse-free and overall survival among patients with gastric cancer. The prognostic signature could be applicable to future decisions concerning treatment.
多项微阵列研究已经报道了可以将癌症患者分为不同预后组的 microRNA（miRNA）表达特征。目前还没有研究评估 miRNA 表达模式与胃癌预后之间的关系。在这项研究中，我们开发了一个与胃癌患者生存密切相关的七 miRNA 特征。
通过实时 RT-PCR 分析 100 名胃癌患者的 miRNA 表达谱，这些患者被随机分配到训练集或测试集中。Cox 比例风险回归和风险评分分析用于在训练集中确定一组与分期无关的七 miRNA 特征，该特征可对具有显著不同预后的患者进行分类。通过测试集和 60 名患者的独立队列进一步验证该 miRNA 特征。
我们确定了一个七 miRNA 特征（miR-10b、miR-21、miR-223、miR-338、let-7a、miR-30a-5p、miR-126），用于预测胃癌患者的总生存（p=0.0009）和无复发生存（p=0.0005）。多变量分析表明，风险特征是总生存（HR=3.046；95%CI，1.246 至 7.445，p=0.015）和无复发生存（HR=3.337；95%CI，1.298 至 8.580，p=0.012）的独立预测因子。此外，该七 miRNA 特征在 50 名测试患者和 60 名独立患者的独立队列中得到了验证。
我们的七 miRNA 特征与胃癌患者的无复发生存和总生存密切相关。该预后特征可适用于未来的治疗决策。
