Banting and Best Department of Medical Research, Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto, Ontario, Canada.
Brief Funct Genomics. 2010 Jan;9(1):13-23. doi: 10.1093/bfgp/elp047. Epub 2009 Dec 1.
In the last decade or so, advances in genome-scale technologies have allowed systematic and detailed analysis of gene function. The experimental accessibility of budding yeast makes it a test-bed for technology development and application of new functional genomic tools and resources that pave the way for comparable efforts in higher eukaryotes. In this article, we review advances in reporter screening technology to discover trans-acting regulators of promoters (or cis-elements) of interest in the context of a novel functional genomics approach called Reporter Synthetic Genetic Array (R-SGA) analysis. We anticipate that this methodology will enable researchers to collect quantitative data on hundreds of gene expression pathways in an effort to better understand transcriptional regulatory networks.
在过去的十年左右，基因组规模技术的进步使得对基因功能进行系统和详细的分析成为可能。出芽酵母的实验可及性使其成为技术开发的试验台，并应用新的功能基因组工具和资源，为在高等真核生物中进行类似的工作铺平道路。在本文中，我们回顾了报告基因筛选技术的进展，以发现启动子（或顺式元件）的反式作用调节剂，这是一种称为报告合成遗传阵列（R-SGA）分析的新型功能基因组学方法的背景下。我们预计，这种方法将使研究人员能够收集数百个基因表达途径的定量数据，努力更好地理解转录调控网络。
