School of Sport and Exercise Sciences, Loughborough University, Loughborough, United Kingdom.
Med Sci Sports Exerc. 2010 Apr;42(4):754-61. doi: 10.1249/MSS.0b013e3181bf94d6.
This study examined the influence of a supplement matrix on the excretion pattern of nandrolone metabolites in response to ingestion of a trace amount of 19-norandrostenedione.
Ten male and nine female volunteers (mean ± SD: age = 26 ± 3 yr, height = 1.71 ± 0.09 m, body mass = 70.9 ± 13.2 kg) were recruited. On two occasions, subjects entered the laboratory in the morning after an overnight fast. After an initial urine collection, subjects ingested either 500 mL of plain water or a commercially available energy bar; 10 µg of 19-norandrostenedione was added to each. The volume of each urine sample passed during the next 24 h was measured, and an aliquot was retained for analysis. All samples were analyzed for the metabolites 19-norandrosterone (19-NA) and 19-noretiocholanolone (19-NE) by gas chromatography-mass spectrometry.
The total volume of urine passed was higher in the water trial (2.10 ± 0.52 L) than in the bar trial (1.85 ± 0.55 L; P = 0.040). Baseline urinary 19-NA concentrations were all below the limit of quantification for the assay. Peak urinary 19-NA was lower (P = 0.002) in the water trial (4.80 ± 2.84 ng·mL(-1)) than in the bar trial (8.46 ± 4.44 ng·mL(-1)). The time elapsed between ingestion of the supplement and the peak urinary 19-NA concentration was longer (P = 0.023) on the bar trial (4.6 ± 2.4 h) than on the water trial (2.8 ± 1.9 h). There was no difference in the total recovery of 19-NA + 19-NE between the liquid and solid supplements (water 30 ± 10%; bar 28 ± 12%; P < 0.140).
Peak 19-NA concentrations were higher, and occurred later, when the 19-norandrostenedione was added to a solid supplement. This may be due to a slower rate of absorption and/or a reduced diuresis, resulting in a longer period for the metabolites to accumulate in the urine.
本研究考察了补充基质对摄入痕量 19-去甲雄烯二酮后，去甲雄烯二酮代谢物排泄模式的影响。
招募了 10 名男性和 9 名女性志愿者（均值±标准差：年龄=26±3 岁，身高=1.71±0.09m，体重=70.9±13.2kg）。两次实验中，志愿者均在禁食过夜后于上午进入实验室。在初始尿液收集后，志愿者分别摄入 500mL 白开水或市售能量棒；每个均添加 10µg 19-去甲雄烯二酮。接下来 24 小时内通过的尿液量被测量，然后保留尿液样本进行分析。所有样本均通过气相色谱-质谱法分析代谢产物 19-雄烯酮（19-NA）和 19-去甲雄烷醇酮（19-NE）。
水试验（2.10±0.52L）的总尿量高于棒试验（1.85±0.55L；P=0.040）。基础尿液 19-NA 浓度均低于检测方法的定量下限。水试验（4.80±2.84ng·mL-1）的尿液 19-NA 峰值低于棒试验（8.46±4.44ng·mL-1）（P=0.002）。棒试验（4.6±2.4h）比水试验（2.8±1.9h）摄入补充剂后达到 19-NA 峰值的时间更长（P=0.023）。液体和固体补充剂中 19-NA+19-NE 的总回收率无差异（水 30±10%；棒 28±12%；P<0.140）。
当 19-去甲雄烯二酮添加到固体补充剂中时，19-NA 峰值更高，且出现时间更晚。这可能是由于吸收速度较慢和/或利尿减少，导致代谢物在尿液中积累的时间更长。
