Sezione di Biologia e Genetica, Dipartimento di Biopatologia e Metodologie Biomediche, Università di Palermo, Via Divisi 83, 90133 Palermo, Italy.
Biotechnol Lett. 2010 Apr;32(4):565-70. doi: 10.1007/s10529-009-0180-5. Epub 2009 Dec 2.
Parietaria judaica pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature Parietaria judaica pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from Parietaria judaica allergic patients we obtained a more detailed characterization of Parietaria judaica allergen profile so to improve our comprehension of the pathogenesis of pollen-induced allergic reaction.
豚草花粉是地中海地区气道过敏性疾病的常见病因。应用二维凝胶电泳（2-DE）和质谱技术对成熟豚草花粉进行蛋白质组分析，建立了该杂草的第一张参考蛋白质组图谱。检测到涉及多种细胞功能以及过敏原发生的蛋白质。通过使用 2-DE 和来自豚草花粉过敏患者的血清进行免疫印迹，我们获得了豚草花粉过敏原谱的更详细特征，从而加深了对花粉诱导过敏反应发病机制的理解。
