Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, UPM, Madrid, España.
Int Arch Allergy Immunol. 2011;156(3):291-6. doi: 10.1159/000323503. Epub 2011 Jun 29.
Cross-reactivity among plant food allergens belonging to the nonspecific lipid transfer protein (LTP) family is well known. In contrast, the relationship among these allergens and their putative homologs from olive (Ole e 7) and Parietaria (Par j 1) pollen has not been clarified.
Sera with specific IgE to LTP allergens were obtained from peach-, mustard- and olive pollen-allergic patients. Purified LTP allergens from foods (peach, apple, mustard and wheat) and pollens (olive, mugwort and Parietaria) were tested by ELISA and ELISA-inhibition assays.
Plant food LTP-allergic patients showed a significantly higher number of sera (89-100 vs. 33-64%) with specific IgE and mean specific IgE levels (0.30-1.56 vs. 0.21-0.34 OD units) to the 4 food LTP allergens tested than to olive Ole e 7 and Parietaria Par j 1 pollen. ELISA-inhibition assays indicated cross-inhibition between food LTP allergens but no cross-reactivity between these allergens and Ole e 7 and Par j 1, or, even more, between the LTP allergens from olive and Parietaria pollen.
LTP allergens from olive and Parietaria pollen cross-react neither with allergenic LTPs from plant foods nor between themselves. Therefore, both pollens do not seem to be related with the LTP syndrome.
属于非特异性脂质转移蛋白（LTP）家族的植物食物过敏原之间的交叉反应是众所周知的。相比之下，这些过敏原与橄榄（Ole e 7）和豚草（Par j 1）花粉中它们的假定同源物之间的关系尚未阐明。
从桃、芥末和橄榄花粉过敏患者中获得了针对 LTP 过敏原的特异性 IgE 血清。通过 ELISA 和 ELISA 抑制试验检测了来自食物（桃、苹果、芥末和小麦）和花粉（橄榄、艾蒿和豚草）的纯化 LTP 过敏原。
植物性食物 LTP 过敏患者的特异性 IgE 血清数量（89-100%对 33-64%）和平均特异性 IgE 水平（0.30-1.56 对 0.21-0.34 OD 单位）对 4 种食物 LTP 过敏原的测试明显高于对橄榄 Ole e 7 和豚草 Par j 1 花粉。ELISA 抑制试验表明食物 LTP 过敏原之间存在交叉抑制，但这些过敏原与 Ole e 7 和 Par j 1 之间无交叉反应，甚至在橄榄和豚草花粉的 LTP 过敏原之间也无交叉反应。
橄榄和豚草花粉的 LTP 过敏原既与植物性食物的致敏性 LTP 无交叉反应，也与彼此之间无交叉反应。因此，这两种花粉似乎都与 LTP 综合征无关。
