National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095 Nanjing, People's Republic of China.
Biotechnol Lett. 2010 Apr;32(4):557-64. doi: 10.1007/s10529-009-0178-z. Epub 2009 Dec 2.
O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, OASTL activity and cysteine levels increased significantly in transgenic plants. Cadmium accumulation and the activity of both superoxide dismutase and catalase enzymes were enhanced in transformants. These results demonstrate that overexpression of GmOASTL4 in tobacco can enhance cysteine levels and increase tolerance to cadmium stress.
O-乙酰丝氨酸（巯基）裂解酶（OASTL，EC 2.5.1.47）催化半胱氨酸生物合成的最后一步。在烟草中过表达大豆 GmOASTL4 基因。与野生型相比，转基因植物的转录本积累明显增加，半胱氨酸含量也更高。在镉胁迫下，转基因植物的 OASTL 活性和半胱氨酸水平显著升高。转化体中镉的积累以及超氧化物歧化酶和过氧化氢酶的活性均增强。这些结果表明，在烟草中过表达 GmOASTL4 可以提高半胱氨酸水平并增强对镉胁迫的耐受性。
