Zhang Ran, Liu Qin, Xu Xiangting, Liao Ming'an, Lin Lijin, Hu Rongping, Luo Xian, Wang Zhihui, Wang Jin, Deng Qunxian, Liang Dong, Xia Hui, Lv Xiulan, Tang Yi, Wang Xun
College of Horticulture, Sichuan Agricultural University, Chengdu, China.
Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, China.
Front Plant Sci. 2022 Oct 10;13:1003743. doi: 10.3389/fpls.2022.1003743. eCollection 2022.
Cadmium (Cd) contamination of paddy soil affects safe crop production. This study aimed to evaluate the effects of plant biostimulant amino acid fertilizer on the phytoremediation capability of an emergent accumulator plant R. Br. for Cd-contaminated paddy soils. A pot study was carried out to study the effects of different concentrations of amino acid fertilizer on the Cd accumulation of grown in Cd-contaminated paddy soil. The amino acid fertilizer increased the biomass of . The amino acid fertilizer concentration exhibited a quadratic polynomial regression relationship with the root and shoot biomass. The fertilizer also increased the photosynthetic pigment (chlorophyll and carotenoid) contents, peroxidase (POD; EC 1.11.1.7) activity, and catalase (CAT; EC 1.11.1.6) activity of , but decreased the soluble protein content and had no significant effect on the superoxide dismutase (SOD; EC 1.15.1.1) activity. Furthermore, the amino acid fertilizer increased the Cd content and Cd extraction of . The shoot Cd extraction increased by 29.06%, 63.05%, 77.22%, and 17.40% at 1500-, 1200-, 900-, and 600-fold dilutions of the amino acid fertilizer, respectively, compared with the control. Moreover, the amino acid fertilizer promoted the Cd transport from the roots to shoots of . The amino acid fertilizer concentration also exhibited a quadratic polynomial regression relationship with the root Cd content, shoot Cd content, root Cd extraction, and shoot Cd extraction, respectively. The correlation, grey relational, and path analyses revealed that the root biomass, shoot biomass, chlorophyll content, catalase activity, shoot Cd content, and root Cd extraction were closely associated with the shoot Cd extraction. Therefore, the amino acid fertilizer can promote Cd uptake and improve the phytoremediation capability of to remediate Cd-contaminated paddy soils, and 900-fold dilution is the most suitable concentration.
稻田土壤中的镉（Cd）污染会影响作物安全生产。本研究旨在评估植物生物刺激素氨基酸肥料对挺水富集植物李氏禾修复镉污染稻田土壤能力的影响。开展了一项盆栽试验，研究不同浓度氨基酸肥料对生长在镉污染稻田土壤中的李氏禾镉积累的影响。氨基酸肥料增加了李氏禾的生物量。氨基酸肥料浓度与根和地上部生物量呈现二次多项式回归关系。该肥料还增加了李氏禾的光合色素（叶绿素和类胡萝卜素）含量、过氧化物酶（POD；EC 1.11.1.7）活性和过氧化氢酶（CAT；EC 1.11.1.6）活性，但降低了可溶性蛋白质含量，且对超氧化物歧化酶（SOD；EC 1.15.1.1）活性无显著影响。此外，氨基酸肥料增加了李氏禾的镉含量和镉提取量。与对照相比，氨基酸肥料1500倍、1200倍、900倍和600倍稀释液处理下地上部镉提取量分别增加了29.06%、63.05%、77.22%和17.40%。此外，氨基酸肥料促进了镉从李氏禾根部向地上部的转运。氨基酸肥料浓度分别与根镉含量、地上部镉含量、根镉提取量和地上部镉提取量也呈现二次多项式回归关系。相关性分析、灰色关联分析和通径分析表明，根生物量、地上部生物量、叶绿素含量、过氧化氢酶活性、地上部镉含量和根镉提取量与地上部镉提取量密切相关。因此，氨基酸肥料可促进李氏禾对镉的吸收，提高其修复镉污染稻田土壤的植物修复能力，900倍稀释液是最合适的浓度。
