Institute of Crop Sciences and National Center for Plant Gene Research, Chinese Academy of Agricultural Sciences, Southern Street of Zhongguancun 12, 100081 Beijing, China.
Plant Mol Biol. 2010 Mar;72(4-5):407-21. doi: 10.1007/s11103-009-9579-6. Epub 2009 Dec 2.
Drought stress greatly affects plant growth and crop yield. To understand the transcriptome dynamics during drought stress in maize seedlings, genome-wide gene expression profiling was compared between the drought-tolerant line Han21 and drought-sensitive line Ye478 using Affymetrix Maize Genome Array containing 17,555 probe sets. The results showed that in response to drought, the Han21 line had fewer probe sets with significant expression change than the Ye478 line and both lines had a common set of ~2,600 regulated probe sets under drought stress. The potential components of the abscisic acid signaling pathway were significantly identified from the common probe sets. A total of 827 probe sets with significantly differential expression between the two lines under drought stress were identified. The differential expression levels of cell wall-related and transporter genes may contribute to the different tolerances of the two lines. Additionally, we found that, compared to the sensitive line Ye478, the transcriptional levels of drought-responsive probe sets in the tolerant line Han21 recovered more quickly after re-watering, and more probe sets in the tolerant line Han21 were exclusively up-regulated at the re-watering stage. Our study provides a global gene expression dynamics of two maize inbred lines during drought stress and re-watering and will be valuable for further study of the molecular mechanisms of drought tolerance in maize.
干旱胁迫严重影响植物生长和作物产量。为了了解玉米幼苗在干旱胁迫下的转录组动态，我们使用包含 17555 个探针的 Affymetrix Maize Genome Array 比较了耐旱系 Han21 和干旱敏感系 Ye478 之间的全基因组基因表达谱。结果表明，在干旱胁迫下，Han21 系中具有显著表达变化的探针数比 Ye478 系少，而且两条系都有一个共同的约 2600 个受调控的探针集。从共同的探针集中显著鉴定出脱落酸信号途径的潜在成分。在干旱胁迫下，两条系之间共鉴定出 827 个差异表达的探针集。细胞壁相关和转运基因的差异表达水平可能导致两条系的不同耐受性。此外，我们发现，与敏感系 Ye478 相比，耐旱系 Han21 中干旱响应探针集的转录水平在重新浇水后恢复得更快，耐旱系 Han21 中更多的探针集在重新浇水阶段被特异性地上调。我们的研究提供了两个玉米自交系在干旱胁迫和再浇水过程中的全基因表达动态，这将有助于进一步研究玉米耐旱的分子机制。
