Xu Feng-Ling, Yao Jun, Ding Mei, Shi Zhang-Sen, Wu Xue, Zhang Jing-Jing, Wang Bao-Jie
a School of Forensic Medicine , China Medical University , Shenyang , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Mar;29(2):250-255. doi: 10.1080/24701394.2016.1275597. Epub 2017 Jan 17.
This study characterized the genetic variations of mitochondrial DNA (mtDNA) to elucidate the maternal genetic structure of Liaoning Han Chinese. A total of 317 blood samples of unrelated individuals were collected for analysis in Liaoning Province. The mtDNA samples were analyzed using two distinct methods: sequencing of the hypervariable sequences I and II (HVSI and HVSII), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the coding region. The results indicated a high gene diversity value (0.9997 ± 0.0003), a high polymorphism information content (0.99668) and a random match probability (0.00332). These samples were classified into 305 haplotypes, with 9 shared haplotypes. The most common haplogroup was D4 (12.93%). The principal component analysis map, the phylogenetic tree map, and the genetic distance matrix all indicated that the genetic distance of the Liaoning Han population from the Tibetan group was distant, whereas that from the Miao group was relatively close.
本研究对线粒体DNA（mtDNA）的遗传变异进行了特征分析，以阐明辽宁汉族的母系遗传结构。在辽宁省共收集了317份无关个体的血样用于分析。mtDNA样本采用两种不同方法进行分析：高变序列I和II（HVSI和HVSII）测序，以及编码区的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析。结果显示基因多样性值较高（0.9997±0.0003）、多态性信息含量较高（0.99668）以及随机匹配概率为（0.00332）。这些样本被分为305个单倍型，其中有9个共享单倍型。最常见的单倍群是D4（12.93%）。主成分分析图、系统发育树图和遗传距离矩阵均表明，辽宁汉族群体与藏族群体的遗传距离较远，而与苗族群体的遗传距离相对较近。
