Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
Proteomics. 2010 Feb;10(4):717-30. doi: 10.1002/pmic.200900462.
The entire complement of proteins expressed by the genome of an organism under a set of defined conditions represents the proteome. The need for rapid, efficient, and high-throughput technologies to understand the proteome of organisms have resulted in the development of protein microarrays. Traditional, cell-based technologies for the generation of protein arrays involve laborious and protracted procedures, which also pose several other technical problems. These limitations have been suitably overcome by cell-free expression systems that carry out rapid, in situ synthesis of proteins from their corresponding DNA templates. This article provides an overview of commonly used cell-free systems that have been used for the generation of protein microarrays, with special emphasis on the diverse applications of cell-free synthesis-based protein microarrays.
在一组定义的条件下，生物体基因组表达的全部蛋白质组成了蛋白质组。为了快速、高效、高通量地了解生物体的蛋白质组，需要开发蛋白质微阵列。传统的基于细胞的蛋白质阵列生成技术涉及繁琐和冗长的步骤，还存在其他一些技术问题。这些限制已经通过无细胞表达系统得到了适当的克服，该系统可以从其相应的 DNA 模板中快速、原位合成蛋白质。本文概述了常用于生成蛋白质微阵列的常用无细胞系统，特别强调了无细胞合成的蛋白质微阵列的各种应用。
