Lu Linli, Lin Bicheng
Institute of Nephrology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Dec;27(6):1397-400, 1409.
The major bottle-neck in the way of constructing high density protein microarray is the availability and stability of proteins. The traditional methods of generating protein arrays require the in-vivo expression, purification and immobilization of hundreds or thousands of proteins. The cell-free protein array technology employs cell-free expression systems to produce proteins directly onto surface from co-distributed or pre-arrayed DNA or RNA, thus avoiding the laborious and often costly processes of protein preparation in the traditional approach. Here we provide an overview of recently developed novel technology in cell free based protein microarray and their applications in protein interaction analysis, in antibody specificity and vaccine screening, and in biomarker assay.
构建高密度蛋白质微阵列的主要瓶颈在于蛋白质的可得性和稳定性。传统的生成蛋白质阵列的方法需要对成百上千种蛋白质进行体内表达、纯化和固定。无细胞蛋白质阵列技术利用无细胞表达系统，直接从共分布或预排列的DNA或RNA在表面产生蛋白质，从而避免了传统方法中蛋白质制备的繁琐且往往成本高昂的过程。在这里，我们概述了基于无细胞的蛋白质微阵列中最近开发的新技术及其在蛋白质相互作用分析、抗体特异性和疫苗筛选以及生物标志物检测中的应用。
