Zentrallabor für Proteinanalytik, Biomedical Center, Ludwig-Maximillians-University of Munich, Munich, Germany.
Proteomics. 2010 Feb;10(4):858-72. doi: 10.1002/pmic.200900609.
In the last decade, proteomic technologies have been increasingly used in fish biology research. Proteomics has been applied primarily to investigate the physiology, development biology and the impact of contaminants in fish model organisms, such as zebrafish (Danio rerio), as well as in some commercial species produced in aquaculture, mainly salmonids and cyprinids. However, the lack of previous genetic information on most fish species has been a major drawback for a more general application of the different proteomic technologies currently available. Also, many teleosts of interest in biological research and with potential application in aquaculture hold unique physiological characteristics that cannot be directly addressed from the study of small laboratory fish models. This review describes proteomic approaches that have been used to investigate diverse biological questions in model and non-model fish species. We will also evaluate the current possibilities to integrate fish proteomics with other "omic" approaches, as well as with additional complementary techniques, in order to address the future challenges in fish biology research.
在过去的十年中，蛋白质组学技术在鱼类生物学研究中得到了越来越多的应用。蛋白质组学主要应用于研究鱼类模式生物（如斑马鱼）的生理学、发育生物学和污染物的影响，以及一些水产养殖的商业物种，主要是鲑鱼和鲤鱼。然而，大多数鱼类缺乏先前的遗传信息，这是当前可用的不同蛋白质组学技术更广泛应用的主要障碍。此外，在生物学研究中具有重要意义且在水产养殖中具有潜在应用的许多硬骨鱼类具有独特的生理特性，无法直接从小型实验室鱼类模型的研究中得到解决。本综述描述了用于研究模型和非模型鱼类物种中不同生物学问题的蛋白质组学方法。我们还将评估将鱼类蛋白质组学与其他“组学”方法以及其他补充技术相结合的当前可能性，以应对鱼类生物学研究未来的挑战。
