Gulturk Sefa, Demirkazik Ayse, Kosar Ilkay, Cetin Ali, Dökmetas Hatice S, Demir Tuncer
Department of Physiology, Cumhuriyet University School of Medicine, Sivas, Turkey.
Bioelectromagnetics. 2010 May;31(4):262-9. doi: 10.1002/bem.20557.
We investigated the effect of long-term exposure to modulation magnetic field (MF), insulin, and their combination on blood-brain barrier (BBB) permeability in a diabetic rat model. Fifty-three rats were randomly assigned to one of six groups: sham, exposed to no MF; MF, exposed to MF; diabetes mellitus (DM), DM induced with streptozotocin (STZ); DM plus MF (DMMF); DM plus insulin therapy (DMI); and DM plus insulin therapy plus MF (DMIMF). All the rats underwent Evans blue (EB) measurement to evaluate the BBB 30 days after the beginning of experiments. The rats in MF, DMMF, and DMIMF groups were exposed to MF (B = 5 mT) for 165 min every day for 30 days. Mean arterial blood pressure (MABP), body mass, and serum glucose level of the study rats were recorded. The extravasation of brain EB of the MF, DM, DMMF, DMI, and DMIMF groups was higher than that of the sham group and the extravasation of right hemisphere of the DMIMF group was highest (P < 0.05). The post-procedure body mass of the sham and MF groups were significantly higher than those of the DM and DMMF groups (P < 0.05). In the DM, DMMF, DMI, and DMIMF groups, the baseline glucose was significantly lower than the post-procedure glucose (P < 0.05). DM and MF increase BBB permeability; in combination, they cause more increase in BBB permeability, and insulin decreases their effect on BBB. Improved glucose metabolism may prevent body mass loss and the hypoglycemic effect of MF. DM increases MABP but MF causes no additional effect.
我们在糖尿病大鼠模型中研究了长期暴露于调制磁场（MF）、胰岛素及其组合对血脑屏障（BBB）通透性的影响。53只大鼠被随机分为六组之一：假手术组，未暴露于MF；MF组，暴露于MF；糖尿病（DM）组，用链脲佐菌素（STZ）诱导糖尿病；DM加MF（DMMF）组；DM加胰岛素治疗（DMI）组；以及DM加胰岛素治疗加MF（DMIMF）组。在实验开始30天后，所有大鼠均进行伊文思蓝（EB）测量以评估血脑屏障。MF组、DMMF组和DMIMF组的大鼠每天暴露于MF（B = 5 mT）165分钟，持续30天。记录研究大鼠的平均动脉血压（MABP）、体重和血清葡萄糖水平。MF组、DM组、DMMF组、DMI组和DMIMF组的脑EB外渗高于假手术组，且DMIMF组右半球的外渗最高（P < 0.05）。假手术组和MF组术后体重显著高于DM组和DMMF组（P < 0.05）。在DM组、DMMF组、DMI组和DMIMF组中，基线血糖显著低于术后血糖（P < 0.05）。DM和MF增加血脑屏障通透性；两者联合时，会使血脑屏障通透性增加更多，而胰岛素会降低它们对血脑屏障的影响。改善葡萄糖代谢可能预防体重减轻以及MF的降血糖作用。DM会增加MABP，但MF不会产生额外影响。
