Guan Yi, Zheng Xin-Min, Yang Zhi-Wei, Li Shi-Wen
Department of Urology, Zhongnan Hospital Affiliated to Wuhan University, Wuhan 430071, China.
Zhonghua Yi Xue Za Zhi. 2009 Jul 14;89(26):1858-61.
To investigate the protective effects of L-carnitine upon testicular ischemia-reperfusion injury in rats.
Sprague-Dawley rats were divided into 3 groups (n = 10). In those animals undergoing unilateral testicular torsion, right testes were rotated 720 degrees for 2 h. Sham operated group served as a control group. Torsion group underwent 2 h torsion and saline was injected intraperitoneally at 30 min pre-detorsion. Treatment group underwent similar torsion but L-carnitine (500 mg/kg) was infused intraoperatively. The right testes of 5 animals in each group were excised after 4 h reperfusion for measuring the levels of malondialdehyde (MDA) and heat shock protein 70 (HSP70), evaluation of activities of antioxidant enzyme including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Histopathological changes and germ cell apoptosis indices (AI) were determined at 24 h post-detorsion in right testes of the remaining 5 animals in each group.
The mean number of apoptotic nuclei per tubule cross section and the malondialdehyde level were significantly lower in treatment group as compared with torsion group [AI( 6.87 +/- 2.47) vs (17.13 +/- 3.56), MDA (160 +/- 15) vs (199 +/- 15) nmol/g]. Activities of antioxidant enzyme and the level of HSP70 were significantly higher in treatment group than those in torsion group [SOD (1638 +/- 153) vs (1078 158) U/g, CAT (317 +/- 28) vs (188 +/- 33) U/g, GPx (667 +/- 94) vs (311 +/- 65) U/g, HSP70 (0.87 +/- 0.13) vs (0.25 +/- 0.04)]. The pathological damage of testes in the treatment group was lighter than that in the torsion group (all P < 0.05).
The administration of L-carnitine exerts a beneficial effect upon testicular ischemia-reperfusion injury. This effect may be achieved through an induced expression of HSP70.
探讨左旋肉碱对大鼠睾丸缺血再灌注损伤的保护作用。
将Sprague-Dawley大鼠分为3组（每组n = 10）。在单侧睾丸扭转的动物中，将右侧睾丸旋转720度，持续2小时。假手术组作为对照组。扭转组进行2小时扭转，并在扭转前30分钟腹腔注射生理盐水。治疗组进行类似的扭转，但术中输注左旋肉碱（500 mg/kg）。每组5只动物在再灌注4小时后切除右侧睾丸，以测量丙二醛（MDA）和热休克蛋白70（HSP70）水平，评估抗氧化酶包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性。每组其余5只动物在扭转后24小时测定右侧睾丸的组织病理学变化和生殖细胞凋亡指数（AI）。
与扭转组相比，治疗组每小管横截面上凋亡核的平均数和丙二醛水平显著降低[AI（6.87±2.47）对（17.13±3.56），MDA（160±15）对（199±15）nmol/g]。治疗组抗氧化酶活性和HSP70水平显著高于扭转组[SOD（1638±153）对（1078±158）U/g，CAT（317±28）对（188±33）U/g，GPx（667±94）对（311±65）U/g，HSP70（0.87±0.13）对（0.25±0.04）]。治疗组睾丸的病理损伤比扭转组轻（所有P < 0.05）。
给予左旋肉碱对睾丸缺血再灌注损伤具有有益作用。这种作用可能通过诱导HSP70表达来实现。
