Ying-feng Liu, Xiao-jing Wang, Qing-yu Wang, Xiao Zhang, Jun Wang
Department of Pediatric Dentistry, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2009 Jun;44(6):355-9.
To establish the three-dimensional images of rat's alveolar bone and to evaluate the effects of nicotine on alveolar bone loss during the process of ligature-induced periodontitis with micro-computed tomography (micro-CT).
Thirty-six adult male SD rats received silk ligatures around the cervix of the right second maxillary molars. Then the animals were randomly assigned to three groups and received daily intraperitoneal injections as follows: group A (control), saline solution; group B, nicotine, 0.83 mg x kg(-1) x d(-1); and group C, nicotine, 1.67 mg x kg(-1) x d(-1). Six animals in each group were randomly selected and sacrificed at day 14 and 28. Micro-CT examinations were used to evaluate the periodontal breakdown.
With the nicotine dose increased, bone mineral density (BMD), bone volume fraction (BVF), and trabecular thickness (TT) gradually reduced, while the trabecular spacing (TS) and alveolar bone loss (ABL) increased. At day 28, the ABL of group C (left, right) was (0.61 +/- 0.14) mm and (1.39 +/- 0.09) mm, and significantly higher than that of group B [(0.39 +/- 0.10) mm and (1.31 +/- 0.06) mm] and group A[(0.30 +/- 0.06) mm and (0.94 +/- 0.07) mm]. The BMD of group C, group B and group A at day 28 was [(617.86 +/- 34.27), (572.46 +/- 31.62) mg/cm3], [(660.04 +/- 36.73), (604.97 +/- 32.59) mg/cm3] and [(709.15 +/- 34.95), (657.04 +/- 30.06) mg/cm3] respectively.
Daily administration of nicotine results in significant bone loss and microstructure deteriorations in the trabeculae of alveolar bone.
建立大鼠牙槽骨的三维图像，并通过显微计算机断层扫描（micro-CT）评估尼古丁在结扎诱导的牙周炎过程中对牙槽骨吸收的影响。
36只成年雄性SD大鼠在右侧上颌第二磨牙颈部用丝线结扎。然后将动物随机分为三组，每天进行腹腔注射，如下：A组（对照组），生理盐水；B组，尼古丁，0.83 mg·kg⁻¹·d⁻¹；C组，尼古丁，1.67 mg·kg⁻¹·d⁻¹。每组随机选取6只动物，在第14天和第28天处死。使用micro-CT检查评估牙周破坏情况。
随着尼古丁剂量增加，骨密度（BMD）、骨体积分数（BVF）和骨小梁厚度（TT）逐渐降低，而骨小梁间距（TS）和牙槽骨吸收（ABL）增加。在第28天，C组（左侧、右侧）的ABL分别为（0.61±0.14）mm和（1.39±0.09）mm，显著高于B组[（0.39±0.10）mm和（1.31±0.06）mm]和A组[（0.30±0.06）mm和（0.94±0.07）mm]。第28天C组、B组和A组的BMD分别为[（617.86±34.27），（572.46±31.62）mg/cm³]，[（660.04±36.73），（604.97±32.59）mg/cm³]和[（709.15±34.95），（657.04±30.06）mg/cm³]。
每日给予尼古丁会导致牙槽骨骨小梁显著骨丢失和微观结构恶化。
