Nociti F H, Nogueira-Filho G R, Primo M T, Machado M A, Tramontina V A, Barros S P, Sallum E A
Department of Prosthodontics and Periodontics, School of Dentistry at Piracicaba, UNICAMP, São Paulo, Brazil.
J Periodontol. 2000 Sep;71(9):1460-4. doi: 10.1902/jop.2000.71.9.1460.
The present study investigated the possible influence of nicotine on the bone loss rate in the furcation region due to ligature-induced periodontitis in rats.
Twenty adult male Wistar rats were included. After anesthesia, the tooth was randomly assigned to receive the cotton ligature in the sulcular area, while the contralateral tooth was left unligated. The animals were randomly assigned to one of the following treatments, including daily intraperitoneal injections: group A, 2 microl/g body weight of saline solution; group B, 2 microl/g body weight of a nicotine solution with 0.13 microl of nicotine/ml of saline solution; group C, 2 microl/g body weight of a nicotine solution with 0.19 microl of nicotine/ml of saline solution; and group D, 2 microl/g body weight of a nicotine solution with 0.26 microl of nicotine/ml of saline solution. Thirty days later, the animals were sacrificed and the specimens routinely processed for serial decalcified sections.
Intergroup analysis revealed greater bone loss in the ligated teeth of group B (1.01 +/- 0.61 mm2), group C (1.14 +/- 0.72 mm2), and group D (1.36 +/- 0.60 mm2) when compared with group A (0.64 +/- 0.62 mm2) (P <0.01). However, no statistically significant differences in bone loss were found among groups B, C, and D. In addition, no bone loss was observed for unligated teeth (P>0.01).
Within the limits of the present study, nicotine enhanced the effects of the local components of periodontal disease in a non-dose-dependent way; nevertheless, the administration of nicotine did not produce periodontal bone loss by itself.
本研究调查了尼古丁对结扎诱导的大鼠牙周炎所致根分叉区骨质流失率的可能影响。
纳入20只成年雄性Wistar大鼠。麻醉后，随机选择一侧牙齿在龈沟区放置棉线结扎，对侧牙齿不结扎。将动物随机分为以下治疗组之一，包括每日腹腔注射：A组，2微升/克体重的生理盐水；B组，2微升/克体重含0.13微升尼古丁/毫升生理盐水的尼古丁溶液；C组，2微升/克体重含0.19微升尼古丁/毫升生理盐水的尼古丁溶液；D组，2微升/克体重含0.26微升尼古丁/毫升生理盐水的尼古丁溶液。30天后，处死动物，标本常规处理制成系列脱钙切片。
组间分析显示，与A组（0.64±0.62平方毫米）相比，B组（1.01±0.61平方毫米）、C组（1.14±0.72平方毫米）和D组（1.36±0.60平方毫米）结扎牙齿的骨质流失更多（P<0.01）。然而，B组、C组和D组之间在骨质流失方面未发现统计学上的显著差异。此外，未结扎牙齿未观察到骨质流失（P>0.01）。
在本研究范围内，尼古丁以非剂量依赖的方式增强了牙周病局部因素的作用；然而，尼古丁本身不会导致牙周骨质流失。
