Ayxiam Hasim, Ma Hong, Ilyar Sheyhidin, Zhang Li-Wei, Ablizi Abudula, Batur Mamtimin, Lu Xiao-Mei
Department of Thoracic Surgery, the First Affiliated Hospital, Xinjiang Medical University, Urumqi, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2009 Jul;43(7):591-6.
To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC.
H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed.
OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC, leucine (0.0043 +/- 0.0006, 0.0040 +/- 0.0006), alanine (0.0039 +/- 0.0007, 0.0033 +/- 0.0006), isoleucine (0.0067 +/- 0.0010, 0.0063 +/- 0.0009), valine (0.0037 +/- 0.0005, 0.0035 +/- 0.0006), glycoprotein (0.0123 +/- 0.0043, 0.0102 +/- 0.0022), lactate (0.0342 +/- 0.0113, 0.0258 +/- 0.0085), acetone (0.0027 +/- 0.0023, 0.0017 +/- 0.0008), acetate (0.0007 +/- 0.0001, 0.0006 +/- 0.0001), choline (0.0035 +/- 0.0006, 0.0029 +/- 0.0007), isobutyrate (0.0020 +/- 0.0004, 0.0018 +/- 0.0003), unsaturated lipid (0.0072 +/- 0.0013, 0.0059 +/- 0.0018), VLDL (0.1209 +/- 0.0589, 0.0879 +/- 0.0269), LDL (0.0885 +/- 0.0328, 0.0785 +/- 0.0288), 1-methylhistidine (0.0005 +/- 0.0001, 0.0004 +/- 0.0005) decreased in EC patient' s plasma with statistical significance (r total > 0.27, P < 0.05), dimethylamine (0.0004 +/- 0.0001, 0.0005 +/- 0.0001), alpha-glucose (0.0079 +/- 0.0013, 0.0081 +/- 0.0016), 3-glucose (0.0139 +/- 0.0024, 0.0142 +/- 0.0029), citric acid (0.0044 +/- 0.0008, 0.0106 +/- 0.0058) increase in the EC patient's plasma (r total < -0.27, P < 0.05). There were clear variation between Han and Kazak patients, alanine (0.0031 +/- 0.0005,0.0029 +/- 0.0004), glutamine (0.0010 +/- 0.0001, 0.0009 +/- 0.0001), tyrosine (0.0009 +/- 0.0001, 0.0008 +/- 0.0001), 1-methylhistidine (0.0005 +/- 0.0001, 0.0004 +/- 0.0001) increased in the Han patients (r > 0.35, P> 0.05), carnitine (0.0028 +/- 0.0006) was higher in Kazak patients than in Han patients (0.0025 +/- 0.0004), which had statistical significance (r = - 0.40, P < 0.05). Unsaturated lipid (0.0059 +/- 0.0018, 0.0047 +/- 0.0011), isoleucine (0.0062 +/- 0.0011, 0.0058 +/- 0.0007), alanine (0. 0032 +/- 0.0007, 0.0028 +/- 0.0004), glycoprotein (0.0096 +/- 0.0019, 0.0086 +/- 0.0011), glutamine (0.0011 +/- 0.0001, 0.0009 +/- 0.0001), tyrosine (0.0009 +/- 0.0001, 0.0008 +/- 0.0001), 1-methylhistidine (0.0005 +/- 0.0001, 0.0004 +/- 0.0001) were increased in Uygur patients as compared with Kazak patients, having statistical significance (r > 0.33, P < 0.05), carnitine (0.0027 +/- 0.0005) was higher in Kazak patients than in Uygur patients (0.0025 +/- 0.0004) (r = - 0.36, P < 0.05).
The results indicate that 1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.
探讨食管癌（EC）患者与健康对照者之间的代谢组学差异，并分析EC患者之间的差异。
对108例EC患者和50例健康对照者的血浆标本进行氢磁共振（H-MR）和正交偏最小二乘法判别分析（OPLS-DA），分析EC患者与健康对照者之间的代谢组学差异。
OPLS-DA分析可正确区分所有健康对照者和EC患者的血浆标本，亮氨酸（0.0043±0.0006，0.0040±0.0006）、丙氨酸（0.0039±0.0007，0.0033±0.0006）、异亮氨酸（0.0067±0.0010，0.0063±0.0009）、缬氨酸（0.0037±0.0005，0.0035±0.0006）、糖蛋白（0.0123±0.0043，0.0102±0.0022）、乳酸（0.0342±0.0113，0.0258±0.0085）、丙酮（0.0027±0.0023，0.0017±0.0008）、乙酸盐（0.0007±0.0001，0.0006±0.0001）、胆碱（0.0035±0.0006，0.0029±0.0007）、异丁酸（0.0020±0.0004，0.0018±0.0003）、不饱和脂质（0.0072±0.0013，0.0059±0.0018）、极低密度脂蛋白（VLDL）（0.1209±0.0589，0.0879±0.0269）、低密度脂蛋白（LDL）（0.0885±0.0328，0.0785±0.0288）、1-甲基组氨酸（0.0005±0.0001，0.0004±0.0005）在EC患者血浆中降低，差异有统计学意义（r总计>0.27，P<0.05），二甲胺（0.0004±0.0001，0.0005±0.0001）、α-葡萄糖（0.0079±0.0013，0.0081±0.0016）、3-葡萄糖（0.0139±0.0024，0.0142±0.0029）、柠檬酸（0.0044±0.0008，0.0106±0.0058）在EC患者血浆中升高（r总计<-0.27，P<0.05）。汉族和哈萨克族患者之间存在明显差异，丙氨酸（0.0031±0.0005，0.0029±0.0004）、谷氨酰胺（0.0010±0.0001，0.0009±0.0001）、酪氨酸（0.0009±0.0001，0.0008±0.0001）、1-甲基组氨酸（0.0005±0.0001，0.0004±0.0001）在汉族患者中升高（r>0.35，P>0.05），肉碱（0.0028±0.0006）在哈萨克族患者中高于汉族患者（0.0025±0.0,004），差异有统计学意义（r=-0.40，P<0.05）。与哈萨克族患者相比，维吾尔族患者的不饱和脂质（0.0059±0.0018，0.0047±0.0011）、异亮氨酸（0.0062±0.0011，0.0058±0.0007）、丙氨酸（0.0032±0.0007，0.0028±0.0004）、糖蛋白（0.0096±0.0019，0.0086±0.0011）、谷氨酰胺（0.0011±0.0001，0.0009±0.0001）、酪氨酸（0.0009±0.0001，0.0008±0.0001）、1-甲基组氨酸（0.0005±0.0001，0.0004±0.0001）升高，差异有统计学意义（r>0.33，P<0.05），肉碱（0.0027±0.0005）在哈萨克族患者中高于维吾尔族患者（0.0025±0.0004）（r=-0.36，P<0.05）。
结果表明，采用OPLS-DA统计方法分析血浆的1H-MR谱可将EC患者与健康对照者完全区分开来。代谢组学方法有助于EC患者的筛查。
