Hasim Ayshamgul, Ali Mayinuer, Mamtimin Batur, Ma Jun-Qi, Li Qiao-Zhi, Abudula Abulizi
Department of Pathology, College of Basic Medicine;
Exp Ther Med. 2012 Jun;3(6):945-951. doi: 10.3892/etm.2012.509. Epub 2012 Mar 12.
(1)H nuclear magnetic resonance (NMR)-based metabonomics has been used to characterize the metabolic profiles of cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC). Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to model the systematic variation related to patients with CIN or CSCC with healthy controls. Potential metabolic biomarkers were identified using database comparisons, and the one-way analysis of variance (ANOVA) test was used to examine the significance of the metabolites. Compared with plasma obtained from the healthy controls, plasma from patients with CIN had higher levels of very-low density lipoprotein (VLDL), acetone, unsaturated lipid and carnitine, together with lower levels of creatine, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine, glycine, acetylcysteine, myo-inositol, choline and glycoprotein. Plasma from patients with CSCC had higher levels of acetate and formate, together with lower levels of creatine, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine and tyrosine compared with the plasma of the healthy controls. In addition, compared with the plasma of patients with CIN, the plasma of CSCC patients had higher levels of acetate, formate, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine, tyrosine, acetylcysteine, myo-inositol, glycoprotein, α-glucose and β-glucose, together with lower levels of acetone, unsaturated lipid and carnitine. Moreover, the profiles showed high feasibility and specificity by statistical analysis with OPLS-DA compared to the Thinprep cytology test (TCT) by setting the histopathological outcome as standard. The metabolic profile obtained for cervical cancer is significant, even for the precancerous disease. This suggests a systemic metabolic response to cancer, which may be used to identify potential early diagnostic biomarkers of the cancer and to establish clinical diagnostic methods.
（1）基于氢核磁共振（NMR）的代谢组学已被用于表征宫颈上皮内瘤变（CIN）和宫颈鳞状细胞癌（CSCC）的代谢谱。主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）被用于构建与CIN或CSCC患者及健康对照相关的系统变异模型。通过数据库比较识别潜在的代谢生物标志物，并使用单因素方差分析（ANOVA）检验来检验代谢物的显著性。与健康对照者的血浆相比，CIN患者血浆中极低密度脂蛋白（VLDL）、丙酮、不饱和脂质和肉碱水平较高，而肌酸、乳酸、异亮氨酸、亮氨酸、缬氨酸、丙氨酸、谷氨酰胺、组氨酸、甘氨酸、乙酰半胱氨酸、肌醇、胆碱和糖蛋白水平较低。与健康对照者的血浆相比，CSCC患者血浆中乙酸盐和甲酸盐水平较高，而肌酸、乳酸、异亮氨酸、亮氨酸、缬氨酸、丙氨酸、谷氨酰胺、组氨酸和酪氨酸水平较低。此外，与CIN患者的血浆相比，CSCC患者血浆中乙酸盐、甲酸盐、乳酸、异亮氨酸、亮氨酸、缬氨酸、丙氨酸、谷氨酰胺、组氨酸、酪氨酸、乙酰半胱氨酸、肌醇、糖蛋白、α-葡萄糖和β-葡萄糖水平较高，而丙酮、不饱和脂质和肉碱水平较低。此外，与薄层液基细胞学检测（TCT）相比，通过将组织病理学结果作为标准进行OPLS-DA统计分析，这些代谢谱显示出较高的可行性和特异性。所获得的宫颈癌代谢谱具有重要意义，即使对于癌前疾病也是如此。这表明对癌症存在全身性代谢反应，这可用于识别癌症潜在的早期诊断生物标志物并建立临床诊断方法。
