Centro de Investigación y Desarrollo en Criotecnología de Alimentos-CIDCA-(CCT La Plata, CONICET-UNLP), Calle 47 Esq. 116. La Plata, Buenos Aires, CP 1900, Argentina.
J Agric Food Chem. 2010 Jan 13;58(1):594-9. doi: 10.1021/jf9029145.
We evaluated the effect of short-term gaseous ozone treatment (10 microL/L; 10 min) on tomato fruit quality and cell wall degradation. The treatments did not modify fruit color, sugar content, acidity, or antioxidant capacity but reduced fruit damage and weight loss and induced the accumulation of phenolic compounds. In addition, softening was delayed in ozone-treated fruit. Cell wall analysis showed that exposure to ozone decreased pectin but not hemicellulose solubilization. Polyuronide depolymerization was also reduced in ozone-treated fruit. While the treatments did not alter the activity of the pectin-degrading enzymes polygalacturonase (PG) and beta-Galactosidase (beta-Gal), a clear decrease in pectin methyl esterase (PME) was found. Results show that short-term ozone treatments might be useful to reduce fruit damage and excessive softening, two of the main factors limiting tomato postharvest life, without negatively affecting other quality attributes. The impact of the treatments on fruit softening might be associated with reduced disassembly (solubilization and depolymerization) of pectic polysaccharides.
我们评估了短期气态臭氧处理（10 微升/升；10 分钟）对番茄果实品质和细胞壁降解的影响。这些处理并未改变果实的颜色、糖含量、酸度或抗氧化能力，但降低了果实损伤和失重，并诱导了酚类化合物的积累。此外，臭氧处理延迟了果实软化。细胞壁分析表明，臭氧暴露降低了果胶但不增加半纤维素的溶解。多聚尿降解也减少了臭氧处理的果实。虽然处理并未改变果胶降解酶多聚半乳糖醛酸酶（PG）和β-半乳糖苷酶（β-Gal）的活性，但发现果胶甲酯酶（PME）明显减少。结果表明，短期臭氧处理可能有助于减少果实损伤和过度软化，这是限制番茄采后寿命的两个主要因素，而不会对其他品质属性产生负面影响。处理对果实软化的影响可能与果胶多糖的解体（溶解和降解）减少有关。
