Verrillo Mariavittoria, Cianciullo Piergiorgio, Cozzolino Vincenza, De Ruberto Francesca, Maresca Viviana, Di Fraia Alessia, Fusaro Lina, Manes Fausto, Basile Adriana
Department of Agricultural Sciences, University of Naples "Federico II", Piazza Carlo di Borbone 1, 80055 Portici, Italy.
Centro Interdipartimentale di Ricerca per la Risonanza Magnetica Nucleare per l'Ambiente, l'Agroalimentare, ed i Nuovi Materiali (CERMANU), University of Naples "Federico II", Piazza Carlo di Borbone 1, 80055 Portici, Italy.
Plants (Basel). 2024 Apr 21;13(8):1154. doi: 10.3390/plants13081154.
The development of new natural antibiotics is considered as the heart of several investigations in the nutraceutical field. In this work, leaves of L. treated by tropospheric ozone (O) and nitrogen (N) deposition, exhibited a clear antimicrobial efficacy against five multi-drug resistant (MDR) bacterial strains (two gram-positive and three gram-negative). Under controlled conditions, it was studied how simulated N deposition influences the response to O and the antibacterial and antioxidant activity, and antioxidant performance. The extraction was performed by ultra-pure acetone using two different steps. A higher antioxidant activity was measured in the presence of interaction between O and N treatments on leaves. At the same time, all organic extracts tested have shown bacteriostatic activity against all the tested strains with a MIC comprised between 9 and 4 micrograms/mL, and a higher antioxidant efficacy shown by spectrophotometric assay. Stronger antimicrobial activity was found in the samples treated with O, whereas N-treated plants exhibited an intermediate antibacterial performance. This performance is related to the stimulation of the non-enzymatic antioxidant system induced by the oxidative stress, which results in an increase in the production of antimicrobial bioactive compounds.
新型天然抗生素的研发被视为营养保健品领域多项研究的核心。在这项工作中，经对流层臭氧（O）和氮（N）沉降处理的L.叶片，对五种多重耐药（MDR）细菌菌株（两种革兰氏阳性菌和三种革兰氏阴性菌）表现出明显的抗菌效果。在受控条件下，研究了模拟氮沉降如何影响对臭氧的响应以及抗菌和抗氧化活性及抗氧化性能。采用超纯丙酮分两步进行提取。在臭氧和氮处理对叶片的相互作用存在时，测得较高的抗氧化活性。同时，所有测试的有机提取物对所有测试菌株均表现出抑菌活性，最低抑菌浓度（MIC）在9至4微克/毫升之间，并且通过分光光度法测定显示出较高的抗氧化功效。在经臭氧处理的样品中发现更强的抗菌活性，而经氮处理的植物表现出中等抗菌性能。这种性能与氧化应激诱导的非酶抗氧化系统的刺激有关，这导致抗菌生物活性化合物的产量增加。
