Spolarics Z, Bautista A P, Spitzer J J
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112.
Hepatology. 1991 Feb;13(2):277-81.
This study elucidates the in vivo metabolic response of different liver cells after a single phagocytic challenge. In vivo glucose uptake of different tissues and isolated liver cells was determined by a sequential double labeling version of the 2-deoxyglucose technique. After latex administration, glucose uptake more than doubled in the liver, increased by about 50% in the spleen and lung and was not changed in muscle and testis. Within 10 min after intravenous injection of latex beads, neutropenia developed, with no change in the number of lymphocytes. This was accompanied by a marked influx of polymorphonuclear leukocytes into the liver. Latex was found in 52%, 35%, and 14% of the isolated Kupffer cells, polymorphonuclear leukocytes and endothelial cells, respectively. In vivo glucose uptake increased by 111%, 142%, and 43% in these cells. Glucose uptake by the latex-free hepatocytes was also elevated, presumably by way of intercellular signals between parenchymal and non-parenchymal liver cells. Indomethacin pretreatment resulted in the delay of neutrophil immigration into tissues without any change in the glucose response of different liver cells. Thus phagocytic stimulation in vivo results in marked neutropenia, migration of neutrophils into the liver, increased glucose uptake by phagocytic cells of the liver and enhanced glucose metabolism by the non-phagocytic parenchymal cells.
本研究阐明了单次吞噬刺激后不同肝细胞的体内代谢反应。通过2-脱氧葡萄糖技术的序贯双标记版本测定不同组织和分离的肝细胞的体内葡萄糖摄取。给予乳胶后，肝脏中的葡萄糖摄取增加了一倍多，脾脏和肺中的葡萄糖摄取增加了约50%，而肌肉和睾丸中的葡萄糖摄取没有变化。静脉注射乳胶珠后10分钟内，出现中性粒细胞减少，淋巴细胞数量无变化。这伴随着多形核白细胞大量涌入肝脏。在分离的库普弗细胞、多形核白细胞和内皮细胞中，分别有52%、35%和14%发现了乳胶。这些细胞中的体内葡萄糖摄取分别增加了111%、142%和43%。无乳胶肝细胞的葡萄糖摄取也升高，可能是通过实质和非实质肝细胞之间的细胞间信号实现的。吲哚美辛预处理导致中性粒细胞向组织内迁移延迟，而不同肝细胞的葡萄糖反应无任何变化。因此，体内吞噬刺激导致明显的中性粒细胞减少、中性粒细胞向肝脏迁移、肝脏吞噬细胞的葡萄糖摄取增加以及非吞噬实质细胞的葡萄糖代谢增强。
