Spolarics Z, Schuler A, Bagby G J, Lang C H, Mészáros K, Spitzer J J
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112.
J Leukoc Biol. 1991 Mar;49(3):309-12. doi: 10.1002/jlb.49.3.309.
This study aims to elucidate the in vivo metabolic response of different liver cells following a short-term (30 min) infusion of a nonlethal dose of human recombinant tumor necrosis factor (TNF). In vivo glucose uptake of different tissues and isolated liver cells was determined by a sequential double-labeling version of the tracer 2-deoxyglucose technique. Following TNF administration glucose uptake was increased in the liver, lung, spleen, and skin while it was not changed in muscle and testis. In response to TNF infusion neutropenia developed which was sustained for 40 min. The number of lymphocytes in the blood was also decreased after the termination of TNF infusion. This short-term infusion of TNF, however, was not accompanied by marked sequestration of leukocytes into the liver. In vivo glucose uptake in response to TNF was doubled in the Kupffer cells and increased by 56% in hepatic endothelial cells. Glucose uptake of parenchymal cells was not significantly affected. The prompt increase of glucose uptake in the reticuloendothelial cells of the liver, primarily in the Kupffer cells, following TNF administration suggests that a similar metabolic response of these cells to sepsis may be mediated at least in part by TNF. It is suggested that the increased glucose uptake by the hepatic nonparenchymal cells is a reflection of the immunomodulatory effect of TNF.
本研究旨在阐明在短期（30分钟）输注非致死剂量的人重组肿瘤坏死因子（TNF）后，不同肝细胞的体内代谢反应。采用示踪剂2-脱氧葡萄糖技术的序贯双标记法测定不同组织和分离肝细胞的体内葡萄糖摄取。给予TNF后，肝脏、肺、脾脏和皮肤的葡萄糖摄取增加，而肌肉和睾丸的葡萄糖摄取未发生变化。TNF输注后出现中性粒细胞减少，并持续40分钟。TNF输注终止后，血液中的淋巴细胞数量也减少。然而，这种短期输注TNF并未伴有白细胞明显滞留于肝脏。TNF刺激下，库普弗细胞的体内葡萄糖摄取增加一倍，肝内皮细胞的葡萄糖摄取增加56%。实质细胞的葡萄糖摄取未受到显著影响。给予TNF后，肝脏网状内皮细胞（主要是库普弗细胞）的葡萄糖摄取迅速增加，这表明这些细胞对脓毒症的类似代谢反应可能至少部分由TNF介导。提示肝脏非实质细胞葡萄糖摄取增加是TNF免疫调节作用的一种体现。
