Yuan Xiao-dong, Wang Shu-juan, Xu Ya-ru, Gao Jie, Yang Na, Li Jing, Li Hong-fen
Department of Neurology, Kailuan Hospital, North China Coal Medical College, Tangshan 063000, China.
Zhonghua Xue Ye Xue Za Zhi. 2009 Sep;30(9):582-7.
To study the distribution characteristics of Beta-fibrinogen (Fg)B gene-854G/A, -455G/A, -249C/T, -148C/T, 448G/A and Bcl-1 G/A polymorphism in North China Han population, and the influence on plasma Fg concentration and molecular reactivity. Further more, to explore the role of Fg gene polymorphisms combining with multi-physiological and environmental factors in the development of cerebral infarction.
Cluster sampling, health examination and questionnaires surveys of 1652 subjects from Tangshan Kailuan Group Corporation were conducted. Blood biochemistry, Fg concentration, fibrin monomer polymerized velocity (FMPV), absorbance maximum (Amax) and FMPV/Amax were measured. The six polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism.
In the population, the proportion of the FgB beta-249 T variation allele was 65.49%, while the proportion of the rest loci was predominantly wild type. The significant differences in Fg concentration and FMPV/Amax were found in -854 genotype groups. The Fg concentration in -854GA group was higher than those in GG and AA group. Only the distribution frequencies of FgB beta Bcl-1 A variation allele, GA and AA genotype in the cerebral infarction group were higher than those in non-infarction group, and the prevalence of cerebral infarction in AA genotype group was higher than other groups (the probability value of above-mentioned results were all P<0.01).
FgB beta Bcl-1A allele and variation genotype were susceptible to cerebral infarction. FgB beta-455GA/448G linkage genotype may contribute to the increased plasma Fg concentration. FgB beta-854 was one of the main controlling gene loci for plasma Fg concentration and molecular reactivity.
研究华北汉族人群中β-纤维蛋白原（Fg）B基因-854G/A、-455G/A、-249C/T、-148C/T、448G/A及Bcl-1 G/A多态性的分布特征，以及对血浆Fg浓度和分子反应性的影响。进一步探讨Fg基因多态性与多种生理和环境因素相结合在脑梗死发生发展中的作用。
采用整群抽样方法，对唐山开滦集团公司1652名受试者进行健康体检和问卷调查。检测血液生化指标、Fg浓度、纤维蛋白单体聚合速度（FMPV）、最大吸光度（Amax）及FMPV/Amax。采用聚合酶链反应-限制性片段长度多态性方法检测上述6种多态性。
在该人群中FgBβ-249 T变异等位基因比例为65.49%，其余位点以野生型为主。-854基因型组Fg浓度和FMPV/Amax差异有统计学意义。-854GA组Fg浓度高于GG和AA组。仅脑梗死组FgBβBcl-1 A变异等位基因、GA和AA基因型的分布频率高于非梗死组，AA基因型组脑梗死患病率高于其他组（上述结果概率值均为P<0.01）。
FgBβBcl-1 A等位基因及变异基因型易患脑梗死。FgBβ-455GA/448G连锁基因型可能导致血浆Fg浓度升高。FgBβ-854是血浆Fg浓度和分子反应性的主要控制基因位点之一。
