[白细胞介素-18及白细胞介素-18受体在免疫性血小板减少症患者Th1主导免疫反应中的作用]

[The role of interleukin-18 and interleukin-18 receptor in predominant Th1 immune response of patients with immune thrombocytopenia].

作者信息

Wang Qian, Yang Xiao-Jing, Shan Ning-Ning, Hou Ming, Ji Xue-Bin, Wang Chun-Yan, Zhu Xiao-Juan, Yang Lei, Zhang Xiao-Lin, Peng Jun, Ma Dao-Xin, Shi Yan

机构信息

Department of Clinical Laboratory, Haematology Oncology Centre, Qilu Hospital, Shandong University, Jinan 250012, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2009 Oct;30(10):658-61.

PMID:19954659

Abstract

OBJECTIVE

To evaluate the role of interleukin (IL)-18 and IL-18 receptor (IL-18R) in the predominant Th1 type cytokine response in patients with immune thrombocytopenia (ITP).

METHODS

Fifteen patients with active phase ITP, eighteen in remission and thirteen healthy controls were enrolled in this study. T-bet and GATA-3 mRNA levels in peripheral blood mononucleated cells (PBMNC) were measured by reverse transcriptase polymerase chain reaction (RT-PCR); the plasma IL-18 level by enzyme linked immunosorbent assay (ELISA), the expression of IL-18R on CD3(+) lymphocytes and total lymphocytes by flow cytometry(FCM).

RESULTS

The T-bet mRNA levels in patients with active phase ITP was 3.572 fold as much as that in the controls (P < 0.05), while the GATA-3 mRNA levels were 0.378 fold of that in controls (P < 0.05). The levels of plasma IL-18 and IL-18R on CD3(+) lymphocytes were significantly increased in active phase ITP than in remission phase and controls. There was no difference in ratio of T-bet/GATA-3 between remitted ITP and controls and so was for T-bet mRNA, GATA-3 mRNA, plasma IL-18 and IL-18R on CD3(+) lymphocytes.

CONCLUSION

ITP as a disease of Th1-dominant response there is an unbalance between T-bet and GATA-3 in its active phase; IL-18 and IL-18R being upregulated.

摘要

目的

评估白细胞介素（IL）-18及IL-18受体（IL-18R）在免疫性血小板减少症（ITP）患者以Th1型细胞因子为主的免疫反应中的作用。

方法

本研究纳入15例活动期ITP患者、18例缓解期患者及13名健康对照者。采用逆转录聚合酶链反应（RT-PCR）检测外周血单个核细胞（PBMNC）中T-bet和GATA-3 mRNA水平；采用酶联免疫吸附测定（ELISA）检测血浆IL-18水平；采用流式细胞术（FCM）检测CD3(+)淋巴细胞及总淋巴细胞上IL-18R的表达。

结果

活动期ITP患者的T-bet mRNA水平是对照组的3.572倍（P < 0.05），而GATA-3 mRNA水平是对照组的0.378倍（P < 0.05）。活动期ITP患者血浆IL-18水平及CD3(+)淋巴细胞上IL-18R的表达显著高于缓解期患者及对照组。缓解期ITP患者与对照组之间T-bet/GATA-3比值无差异，CD3(+)淋巴细胞上T-bet mRNA、GATA-3 mRNA、血浆IL-18及IL-18R水平亦无差异。