Wang Qian, Yang Xiao-Jing, Shan Ning-Ning, Hou Ming, Ji Xue-Bin, Wang Chun-Yan, Zhu Xiao-Juan, Yang Lei, Zhang Xiao-Lin, Peng Jun, Ma Dao-Xin, Shi Yan
Department of Clinical Laboratory, Haematology Oncology Centre, Qilu Hospital, Shandong University, Jinan 250012, China.
Zhonghua Xue Ye Xue Za Zhi. 2009 Oct;30(10):658-61.
To evaluate the role of interleukin (IL)-18 and IL-18 receptor (IL-18R) in the predominant Th1 type cytokine response in patients with immune thrombocytopenia (ITP).
Fifteen patients with active phase ITP, eighteen in remission and thirteen healthy controls were enrolled in this study. T-bet and GATA-3 mRNA levels in peripheral blood mononucleated cells (PBMNC) were measured by reverse transcriptase polymerase chain reaction (RT-PCR); the plasma IL-18 level by enzyme linked immunosorbent assay (ELISA), the expression of IL-18R on CD3(+) lymphocytes and total lymphocytes by flow cytometry(FCM).
The T-bet mRNA levels in patients with active phase ITP was 3.572 fold as much as that in the controls (P < 0.05), while the GATA-3 mRNA levels were 0.378 fold of that in controls (P < 0.05). The levels of plasma IL-18 and IL-18R on CD3(+) lymphocytes were significantly increased in active phase ITP than in remission phase and controls. There was no difference in ratio of T-bet/GATA-3 between remitted ITP and controls and so was for T-bet mRNA, GATA-3 mRNA, plasma IL-18 and IL-18R on CD3(+) lymphocytes.
ITP as a disease of Th1-dominant response there is an unbalance between T-bet and GATA-3 in its active phase; IL-18 and IL-18R being upregulated.
评估白细胞介素（IL）-18及IL-18受体（IL-18R）在免疫性血小板减少症（ITP）患者以Th1型细胞因子为主的免疫反应中的作用。
本研究纳入15例活动期ITP患者、18例缓解期患者及13名健康对照者。采用逆转录聚合酶链反应（RT-PCR）检测外周血单个核细胞（PBMNC）中T-bet和GATA-3 mRNA水平；采用酶联免疫吸附测定（ELISA）检测血浆IL-18水平；采用流式细胞术（FCM）检测CD3(+)淋巴细胞及总淋巴细胞上IL-18R的表达。
活动期ITP患者的T-bet mRNA水平是对照组的3.572倍（P < 0.05），而GATA-3 mRNA水平是对照组的0.378倍（P < 0.05）。活动期ITP患者血浆IL-18水平及CD3(+)淋巴细胞上IL-18R的表达显著高于缓解期患者及对照组。缓解期ITP患者与对照组之间T-bet/GATA-3比值无差异，CD3(+)淋巴细胞上T-bet mRNA、GATA-3 mRNA、血浆IL-18及IL-18R水平亦无差异。
ITP作为一种以Th1为主导反应的疾病，其活动期T-bet与GATA-3之间失衡；IL-18及IL-18R上调。
