Yoshida M, Ikawa Y, Owada M, Toyoshima K
Int J Cancer. 1977 Oct 15;20(4):560-3. doi: 10.1002/ijc.2910200413.
The adenylate cyclase activity was measured in chick embryo fibroblasts (CEF) infected with temperature-sensitive mutants (ts) of avian sarcoma virus (ASV). When CEF transformed with a (ts) mutant at 36 degrees C were incubated at the non-permissive temperature (41 degrees C), recovery from the low adenylate cyclase activity detectable in the transformed state was slower than the disappearance of signs of morphological transformation. After a downward shift of the temperature the activity decreased and this change was also slower than the alteration of cell morphology. The affinity of the enzyme system for ATP also changed after, and not during, morphological alteration. No significant difference was observed between the cAMP levels in ASV-transformed and non-infected CEF. These findings are consistent with the idea that adenylate cyclase is not involved in cell transformation and that the change in its activity is secondary to cell transformation.
用禽肉瘤病毒（ASV）温度敏感突变体（ts）感染鸡胚成纤维细胞（CEF），并检测其腺苷酸环化酶活性。当在36℃用（ts）突变体转化的CEF在非允许温度（41℃）下孵育时，从转化状态下可检测到的低腺苷酸环化酶活性恢复的速度比形态转化迹象的消失要慢。温度下调后，活性降低，这种变化也比细胞形态的改变要慢。酶系统对ATP的亲和力在形态改变之后而非期间也发生了变化。在ASV转化的CEF和未感染的CEF中，cAMP水平未观察到显著差异。这些发现与以下观点一致，即腺苷酸环化酶不参与细胞转化，其活性变化是细胞转化的继发结果。
