Institute of Zoology, National Taiwan University, Taipei, Taiwan.
Vaccine. 2010 Jan 22;28(4):996-1001. doi: 10.1016/j.vaccine.2009.10.132. Epub 2009 Nov 30.
Nervous necrosis virus (NNV) has caused mass mortality in many species of cultured fish at larval stage. Strong evidence of vertical transmission of NNV has been reported in the carrier broodstock of striped jack and sea bass. An effective immunization program was developed and monitored in adult groupers (Epinephelus coioides) with average body weight of 1.35kg. The highest neutralizing antibody titers were found in the fish intramuscularly injected with adjuvanted NNV vaccine at 10(9)TCID(50)kg(-1), and the enhanced 2-fold neutralization activity could sustain up to 17 months post-vaccination (mpv). An immunization program was applied in the broodstocks of grouper (Epinephelus tukula) with body weight of 35-60kg. The levels of NNV-specific antibodies detected, from 1 to 5 mpi, in the homogenates of the eggs from the vaccinated broodfish were elevated than that from non-vaccinated fish. By nested RT-PCR, NNV was detectable in the eggs from the non-vaccinated fish at Month 5, but not in the eggs from vaccinated fish. It is therefore suggested that vaccination will be a potentially practical measure to reduce the risk of vertical transmission of NNV of grouper broodfish under stress of repeated spawning.
神经坏死病毒（NNV）已导致许多幼鱼养殖品种大量死亡。已报道斜带石斑鱼和海鲈的带病毒亲鱼存在垂直传播 NNV 的有力证据。已为体重为 1.35kg 的成年军曹鱼（Epinephelus coioides）制定并监测了有效的免疫计划。在以 10(9)TCID(50)kg(-1)的剂量肌肉内注射佐剂 NNV 疫苗的鱼中发现了最高的中和抗体滴度，增强的 2 倍中和活性可持续至疫苗接种后 17 个月（mpv）。已将免疫计划应用于体重为 35-60kg 的军曹鱼（Epinephelus tukula）亲鱼中。从疫苗接种亲鱼的卵的匀浆中检测到的 NNV 特异性抗体水平（1 至 5 mpi）高于未接种疫苗的鱼。通过巢式 RT-PCR，在未接种疫苗的鱼的卵中可在第 5 个月检测到 NNV，但在接种疫苗的鱼的卵中未检测到。因此，建议在亲鱼反复产卵的应激下，疫苗接种将是减少 NNV 垂直传播风险的一种潜在实用措施。
