University Clinic of Respiratory and Allergic Diseases, Golnik 36, 4204 Golnik, Slovenia.
Respir Med. 2010 Apr;104(4):571-7. doi: 10.1016/j.rmed.2009.11.008. Epub 2009 Dec 1.
Invariant natural killer T (NKT) cells might contribute to the amplified and prolonged T-cell immune response that characterizes sarcoidosis. Therefore, we want to investigate the frequency and distribution of pulmonary invariant NKT cells in corticosteroid-naïve patients with sarcoidosis. We used multi-parameter flow cytometry with antibodies against CD3, CD4, CD8, CD14, CD19, CD45, CD16/56, TCR Valpha24, and TCR Vbeta11, on bronchoalveolar lavage fluid (BALF), to examine the frequency and distribution of pulmonary invariant NKT cells in 47 newly diagnosed sarcoidosis patients and in 8 control subjects. The frequencies of BALF Valpha24 Vbeta11 invariant NKT cells were significantly lower in patients with sarcoidosis in comparison to control subjects. Moreover, lower invariant NKT cell frequencies in patients with sarcoidosis significantly correlated with exaggerated BALF lymphocytosis and CD4 T cell responses. This study demonstrated a pulmonary deficiency in the frequency of a subset of T cells with immunoregulatory function in patients with sarcoidosis.
不变自然杀伤 T (NKT) 细胞可能有助于放大和延长结节病的 T 细胞免疫反应。因此，我们希望研究初治结节病患者肺内不变 NKT 细胞的频率和分布。我们使用多参数流式细胞术，针对 CD3、CD4、CD8、CD14、CD19、CD45、CD16/56、TCR Valpha24 和 TCR Vbeta11，对支气管肺泡灌洗液 (BALF) 进行检测，以研究 47 例新诊断的结节病患者和 8 例对照者肺内不变 NKT 细胞的频率和分布。与对照组相比，结节病患者 BALF Valpha24 Vbeta11 不变 NKT 细胞的频率显著降低。此外，结节病患者不变 NKT 细胞频率降低与 BALF 淋巴细胞增多和 CD4 T 细胞反应过度显著相关。这项研究表明，结节病患者存在具有免疫调节功能的 T 细胞亚群频率降低。
