内源性肽在大鼠生物钟中的发现：利用超高性能串联质谱对视交叉上核的重点研究。
Endogenous peptide discovery of the rat circadian clock: a focused study of the suprachiasmatic nucleus by ultrahigh performance tandem mass spectrometry.
机构信息
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
出版信息
Mol Cell Proteomics. 2010 Feb;9(2):285-97. doi: 10.1074/mcp.M900362-MCP200. Epub 2009 Nov 10.
Abstract
Understanding how a small brain region, the suprachiasmatic nucleus (SCN), can synchronize the body's circadian rhythms is an ongoing research area. This important time-keeping system requires a complex suite of peptide hormones and transmitters that remain incompletely characterized. Here, capillary liquid chromatography and FTMS have been coupled with tailored software for the analysis of endogenous peptides present in the SCN of the rat brain. After ex vivo processing of brain slices, peptide extraction, identification, and characterization from tandem FTMS data with <5-ppm mass accuracy produced a hyperconfident list of 102 endogenous peptides, including 33 previously unidentified peptides, and 12 peptides that were post-translationally modified with amidation, phosphorylation, pyroglutamylation, or acetylation. This characterization of endogenous peptides from the SCN will aid in understanding the molecular mechanisms that mediate rhythmic behaviors in mammals.
摘要
了解一个小的脑区，视交叉上核（SCN），如何同步身体的昼夜节律是一个正在进行的研究领域。这个重要的计时系统需要一整套复杂的肽激素和递质，它们的特征仍不完全清楚。在这里，毛细管液相色谱和 FTMS 已经与定制的软件相结合，用于分析大鼠脑 SCN 中存在的内源性肽。在大脑切片的离体处理后，肽提取、鉴定和从串联 FTMS 数据中进行特征分析，具有<5-ppm 的质量精度，生成了 102 种内源性肽的超置信列表，包括 33 种以前未鉴定的肽，以及 12 种肽被酰胺化、磷酸化、焦谷氨酸化或乙酰化的翻译后修饰。对 SCN 中内源性肽的这种特征描述将有助于理解调节哺乳动物节律行为的分子机制。
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