Suganthi Devadason Marine Research Institute, 44- Beach Road, Tuticorin 628001, Tamil Nadu, India.
Water Sci Technol. 2009;60(12):3225-32. doi: 10.2166/wst.2009.756.
Uncontaminated soil, Vat Blue 4 contaminated soil and Vat Blue 4 effluent were screened for heterotrophic bacterial population and the bacterial density were found to be 19.3 x 10(4) Colony Forming Units (CFU)/gm, 5.5 x 10(4) CFU/gm and 1.1 x 10(4) CFU/ml respectively. Student's 't' test analysis affirmed that significant variation prevailed between the three set of 't' tests conducted (P<0.001 to 0.002). The heterotrophic bacterial population of dye contaminated soil comprised of 32.5% of Pseudomonas spp. followed by 27.5% of Bacillus spp., 15.0% of Aeromonas spp., 12.5% of Micrococcus spp. and 12.5% of Achromobacter spp. The optimum inoculums load, pH and temperature were found to be 5% (10 x 10(4) counts), 10 and 35 degrees C respectively. Free cells of B. subtilis decolorized Vat Blue 4 up to 92.30% after 24 hours of treatment. Total Dissolved Solids (TDS), Biological Oxygen Demand (BOD(5)) and Chemical Oxygen Demand (COD) were reduced up to 50.00, 79.60 and 75.40% respectively.
未受污染的土壤、Vat Blue 4 污染土壤和 Vat Blue 4 废水的异养细菌种群进行了筛选，细菌密度分别为 19.3 x 10(4)菌落形成单位 (CFU)/gm、5.5 x 10(4) CFU/gm 和 1.1 x 10(4) CFU/ml。学生 t 检验分析证实，三组 t 检验之间存在显著差异（P<0.001 至 0.002）。染料污染土壤中的异养细菌种群由 32.5%的假单胞菌属、27.5%的芽孢杆菌属、15.0%的气单胞菌属、12.5%的微球菌属和 12.5%的无色杆菌属组成。最佳接种量、pH 值和温度分别为 5%（10 x 10(4)个细胞）、10 和 35°C。枯草芽孢杆菌的游离细胞在 24 小时的处理后可将 Vat Blue 4 脱色至 92.30%。总溶解固体 (TDS)、生物需氧量 (BOD(5)) 和化学需氧量 (COD) 分别降低了 50.00%、79.60%和 75.40%。
