膜联蛋白 IV 在卵巢透明细胞癌中呈差异性表达。
Annexin IV is differentially expressed in clear cell carcinoma of the ovary.
机构信息
Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, People's Republic of China.
出版信息
Int J Gynecol Cancer. 2009 Dec;19(9):1545-9. doi: 10.1111/IGC.0b013e3181a844c8.
OBJECTIVE
To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray.
METHODS
Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC.
RESULTS
Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray.
CONCLUSIONS
The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.
目的
应用 cDNA 微阵列技术探讨卵巢透明细胞癌（CCC）与浆液性囊腺癌（SAC）之间差异表达的基因。
方法
采用 cDNA 微阵列技术对 8 例卵巢 CCC 组织和 8 例卵巢 SAC 组织进行检测，应用实时聚合酶链反应对差异表达基因进行鉴定和验证，采用免疫组织化学法和 Western blot 法检测 Annexin IV 蛋白在卵巢 CCC 细胞和组织中的表达。
结果
比较基因表达谱发现，卵巢 CCC 与 SAC 之间有 21 个基因的表达差异超过 2 倍，其中上调基因 9 个，下调基因 12 个。细胞系和组织中 Annexin IV 的验证结果与微阵列结果一致。
结论
cDNA 微阵列技术是探讨卵巢透明细胞癌和浆液性囊腺癌基因表达谱差异的一种可行方法。Annexin IV 的过表达可能是卵巢 CCC 的特异性分子标志物。
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