Laboratory of Gastroenterology and Hepatology, Hallym University Medical Center, Seoul, Korea.
Cancer Res Treat. 2005 Aug;37(4):228-32. doi: 10.4143/crt.2005.37.4.228. Epub 2005 Aug 31.
Maspin is known as a tumor suppressor gene, but its significance has been questioned in various human cancers. The aim of this study was to investigate the expression pattern of Maspin in human gastric adenocarcinomas and its possible correlation with clinicopathological findings.
The expression of Maspin mRNA was measured by nested RT-PCR using 60 frozen adenocarcinomas of the stomach and 31 noncancerous tissues from the proximal resection margin. Immunohistochemical study for Maspin protein expression was carried out using 62 paraffin-embedded tissues, composed of both cancer and noncancerous tissues.
Maspin mRNA expression was detected in 80.0% (48 of 60) of the gastric adenocarcinomas, but in only 22.6% (7 of 31) of the normal gastric mucosa (p<0.001). The positive rate of Maspin protein expression was higher in the adenocarcinomas than the normal tissues (62.9% vs. 27.4%, p<0.05). In addition, the intestinal type of tumors showed significantly higher expression levels compared to the diffuse type of tumors (81.5% vs. 48.6%, p<0.05).
Our results suggest that Maspin is frequently expressed in human gastric cancers, and its expression might be associated with tumorigenesis of the intestinal type of gastric cancer.
Maspin 是一种已知的肿瘤抑制基因，但它在各种人类癌症中的意义一直存在争议。本研究旨在探讨 Maspin 在人胃腺癌中的表达模式及其与临床病理发现的可能相关性。
使用 60 例冷冻胃腺癌和 31 例近端切缘非癌组织的嵌套 RT-PCR 测量 Maspin mRNA 的表达。使用 62 例石蜡包埋组织（包括癌组织和非癌组织）进行 Maspin 蛋白表达的免疫组织化学研究。
Maspin mRNA 在 80.0%（60 例中的 48 例）胃腺癌中检测到，但仅在 22.6%（31 例中的 7 例）正常胃黏膜中检测到（p<0.001）。腺癌中 Maspin 蛋白表达的阳性率高于正常组织（62.9%比 27.4%，p<0.05）。此外，肠型肿瘤的表达水平明显高于弥漫型肿瘤（81.5%比 48.6%，p<0.05）。
我们的结果表明，Maspin 在人胃腺癌中频繁表达，其表达可能与肠型胃癌的肿瘤发生有关。
