Son Hee Jung, Sohn Tae-Sung, Song Sang Yong, Lee Jun Haeng, Rhee Jong Chul
Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Pathol Int. 2002 Aug;52(8):508-13. doi: 10.1046/j.1440-1827.2002.01381.x.
Maspin, a member of the serpin family of protease inhibitors, is known to be a tumor suppressor. The relationship between its expression and biological significance in various human cancers has not been elucidated. We studied maspin expression in 30 cases of human gastric adenocarcinoma using immunohistochemistry and reverse transcription-polymerase chain reaction. Twenty-seven cases (90%) of gastric adenocarcinoma, regardless of histological type, and all the cases of gastric epithelial cells with intestinal metaplasia (GECIM) showed diffuse and strong immunoreactivity for maspin. Eighteen of 26 cases (69.2%) of non-metaplastic, non-carcinoma gastric epithelia (NMNCE) showed weak and focal immunoreactivity. The level of maspin expression was higher in the GECIM and lower in the NMNCE than in the adenocarcinoma cases. Maspin mRNA expression was detected in all the samples from non-carcinoma mucosa and gastric adenocarcinoma, and was higher in the gastric adenocarcinoma than in the non-carcinoma mucosa (P < 0.001). The lower carcinoma/non-carcinoma mRNA expression rate correlated to the underexpression of maspin immunohistochemistry (P < 0.001). These data suggest that maspin may contribute to gastric carcinogenesis. In addition, immunohistochemical expression of maspin seems to reflect mRNA expression.
Maspin是丝氨酸蛋白酶抑制剂（serpin）家族的一员，已知其具有肿瘤抑制作用。其在各种人类癌症中的表达与生物学意义之间的关系尚未阐明。我们采用免疫组织化学和逆转录-聚合酶链反应，研究了30例人类胃腺癌中maspin的表达情况。27例（90%）胃腺癌病例，无论组织学类型如何，以及所有肠化生胃上皮细胞（GECIM）病例，对maspin均表现出弥漫性强免疫反应性。26例非化生、非癌性胃上皮（NMNCE）病例中有18例（69.2%）表现出弱的局灶性免疫反应性。GECIM中maspin的表达水平高于腺癌病例，而NMNCE中的表达水平低于腺癌病例。在所有非癌黏膜和胃腺癌样本中均检测到maspin mRNA表达，且胃腺癌中的表达高于非癌黏膜（P < 0.001）。较低的癌/非癌mRNA表达率与maspin免疫组织化学表达不足相关（P < 0.001）。这些数据表明maspin可能参与了胃癌的发生。此外，maspin的免疫组织化学表达似乎反映了mRNA表达。
