Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
PLoS Genet. 2009 Nov;5(11):e1000746. doi: 10.1371/journal.pgen.1000746. Epub 2009 Nov 26.
Epithelial tubes are the functional units of many organs, and proper tube geometry is crucial for organ function. Here, we characterize serrano (sano), a novel cytoplasmic protein that is apically enriched in several tube-forming epithelia in Drosophila, including the tracheal system. Loss of sano results in elongated tracheae, whereas Sano overexpression causes shortened tracheae with reduced apical boundaries. Sano overexpression during larval and pupal stages causes planar cell polarity (PCP) defects in several adult tissues. In Sano-overexpressing pupal wing cells, core PCP proteins are mislocalized and prehairs are misoriented; sano loss or overexpression in the eye disrupts ommatidial polarity and rotation. Importantly, Sano binds the PCP regulator Dishevelled (Dsh), and loss or ectopic expression of many known PCP proteins in the trachea gives rise to similar defects observed with loss or gain of sano, revealing a previously unrecognized role for PCP pathway components in tube size control.
上皮管是许多器官的功能单位，而管腔的正确几何形状对器官功能至关重要。在这里，我们描述了sano（sano），这是一种新型的细胞质蛋白，在果蝇的几种形成管状的上皮组织中，包括气管系统，都在上皮顶部分布丰富。sano 缺失会导致气管伸长，而 Sano 过表达会导致气管变短，顶边界减少。在幼虫和蛹阶段过表达 sano 会导致几个成年组织的平面细胞极性（PCP）缺陷。在过表达 sano 的蛹翅细胞中，核心 PCP 蛋白发生了错误定位，并且预毛发生了错误取向；sano 在眼睛中的缺失或过表达会破坏小眼极性和旋转。重要的是，Sano 与 PCP 调节因子 Dishevelled（Dsh）结合，并且气管中许多已知的 PCP 蛋白的缺失或异位表达会导致与 sano 缺失或获得观察到的相似缺陷，这揭示了 PCP 途径成分在管腔大小控制中的一个以前未被认识到的作用。
