Hitachi Plant Technologies, Ltd., 537 Kami-Hongo, Matsudo, Chiba 271-0064, Japan.
Appl Microbiol Biotechnol. 2010 Mar;86(1):359-65. doi: 10.1007/s00253-009-2359-z. Epub 2009 Dec 3.
In order to assess the stability of nitrogen removal systems utilizing anaerobic ammonium oxidation (anammox), it is necessary to study the toxic effects of nitrite on these biochemical reactions. In this study, the effects of nitrite on anammox bacteria entrapped in gel carriers were investigated using batch and continuous feeding tests. The results showed that the nitrite concentration in a reactor must be less than 274-mg N/L in order to prevent a decrease in the anammox activity, which occurred when the gel carriers were soaked in nitrite solutions with concentrations greater than 274-mg N/L in a batch test. In a continuous feeding test, nitrite inhibition was not observed at low concentrations of nitrite. However, the anammox activity decreased to 10% when the nitrite concentration increased to 750-mg N/L over a 7-day period in the reactor. In addition, it was shown that the effects of nitrogen on the anammox reaction were reversible because the anammox activity completely recovered within 3 days when the influent nitrite concentration was decreased to less than 274-mg N/L.
为了评估利用厌氧氨氧化（anammox）的氮去除系统的稳定性，有必要研究亚硝酸盐对这些生化反应的毒性影响。在这项研究中，使用批式和连续进料试验研究了亚硝酸盐对凝胶载体中固定化 anammox 细菌的影响。结果表明，为了防止 anammox 活性下降，反应器中亚硝酸盐浓度必须小于 274mg N/L，在批式试验中，当凝胶载体浸泡在浓度大于 274mg N/L 的亚硝酸盐溶液中时，会发生 anammox 活性下降的情况。在连续进料试验中，在低浓度的亚硝酸盐下没有观察到亚硝酸盐抑制。然而，当反应器中亚硝酸盐浓度在 7 天内增加到 750mg N/L 时，anammox 活性下降到 10%。此外，研究表明，氮对 anammox 反应的影响是可逆的，因为当进水亚硝酸盐浓度降低到小于 274mg N/L 时，anammox 活性在 3 天内完全恢复。
