Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong, 266071, China.
Mar Biotechnol (NY). 2010 Oct;12(5):558-68. doi: 10.1007/s10126-009-9242-x. Epub 2009 Dec 3.
Half-smooth tongue sole (Cynoglossus semilaevis) is an increasingly important aquaculture species in China. It is also a tractable model to study sex chromosome evolution and to further elucidate the mechanism of sex determination in teleosts. Two bacterial artificial chromosome (BAC) libraries for C. semilaevis, with large, high-quality inserts and deep coverage, were constructed in the BamHI and HindIII sites of the vector pECBAC1. The two libraries contain a total of 55,296 BAC clones arrayed in 144 384-well microtiter plates and correspond to 13.36 haploid genome equivalents. The combined libraries have a greater than 99% probability of containing any single-copy sequence. Screening high-density arrays of the libraries with probes for female-specific markers and sex-related genes generated between 4-46 primary positive clones per probe. Thus, the two BAC libraries of C. semilaevis provided a readily useable platform for genomics research, illustrated by the isolation of sex determination gene(s).
半滑舌鳎（Cynoglossus semilaevis）是中国日益重要的水产养殖品种。它也是研究性染色体进化和进一步阐明硬骨鱼类性别决定机制的一种易于处理的模式生物。构建了两个用于半滑舌鳎的细菌人工染色体（BAC）文库，它们在载体 pECBAC1 的 BamHI 和 HindIII 位点上具有大的、高质量的插入片段和深的覆盖度。这两个文库共包含 55,296 个 BAC 克隆，排列在 144 个 384 孔微孔板中，对应于 13.36 个单倍体基因组当量。组合文库包含任何单拷贝序列的可能性大于 99%。用雌性特异性标记和性别相关基因的探针筛选文库的高密度阵列，每个探针产生 4-46 个初级阳性克隆。因此，这两个半滑舌鳎的 BAC 文库为基因组学研究提供了一个易于使用的平台，例如分离性别决定基因。
