永久遗传资源：从南方鲆（ Paralichthys lethostigma ）中分离和鉴定微卫星 DNA 位点。

PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite DNA loci from the southern flounder, Paralichthys lethostigma.

机构信息

Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):381-3. doi: 10.1111/j.1471-8286.2007.01965.x.

DOI:10.1111/j.1471-8286.2007.01965.x

PMID:21585798

Abstract

Paucity of polymorphic molecular markers in southern flounder (Paralichthys lethostigma) has been a major limitation in genetic improvement of this important economic fish. Hence, we constructed a repeat-enriched genomic library from P. lethostigma. A total of 39 new microsatellites were identified, for which 33 primer pairs were designed. After validating and scoring, 10 of these loci were polymorphic in a test population with the range of alleles from two to nine per locus. The observed and expected heterozygosities ranged from 0.2500 to 0.9000 and from 0.4469 to 0.8514, respectively. These polymorphic microsatellites will be useful for genetic diversity analysis and linkage map construction for P. lethostigma.

摘要

南方鲇（Paralichthys lethostigma）缺乏多态性分子标记，这一直是遗传改良这种重要经济鱼类的主要限制因素。因此，我们从南方鲇中构建了一个重复富集基因组文库。共鉴定出 39 个新的微卫星，为其设计了 33 对引物。经过验证和评分，在一个测试群体中，有 10 个位点具有多态性，每个位点的等位基因数从两个到九个不等。观察到的和预期的杂合度分别在 0.2500 到 0.9000 和 0.4469 到 0.8514 之间。这些多态性微卫星将有助于分析南方鲇的遗传多样性和构建连锁图谱。