Department of Food and Nutrition, Kunsan National University, Kunsan, South Korea.
Phytother Res. 2010 Jun;24(6):834-9. doi: 10.1002/ptr.3027.
The radix of Pueraria thunbergiana (P. thunbergiana) is traditionally prescribed to attenuate the clinical manifestation of inner ear dysfunction and various clinical situations including fevers, gastrointestinal disorders, skin problems, migraine headaches, lowering cholesterol, and treating chronic alcoholism in oriental medicine. In the present study, we examined the protective effect of ethanol extract of the radix of P. thunbergiana (RPT) on cisplatin-induced damage of HEI-OC1 auditory hair cells. When the cells were cultured in the medium containing 5-100 microg/mL of RPT, RPT showed protective effect against the cisplatin-induced HEI-OC1 cell damage. We also measured the effects of RPT on lipid peroxidation of cisplatin-treated cells as well as scavenging activities against superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. RPT reduced cisplatin-induced lipid peroxidation in a dose-dependent manner. Furthermore, RPT showed strong scavenging activity against superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. These results indicate that RPT protects cisplatin-induced HEI-OC1 cell damage through inhibition of lipid peroxidation and scavenging activities of free radials.
野葛的根部在东方医学中被传统用于减轻内耳功能障碍的临床表现和各种临床情况，包括发热、胃肠紊乱、皮肤问题、偏头痛、降低胆固醇和治疗慢性酒精中毒。在本研究中，我们研究了野葛的乙醇提取物（RPT）对顺铂诱导的 HEI-OC1 听觉毛细胞损伤的保护作用。当细胞在含有 5-100μg/mL 的 RPT 的培养基中培养时，RPT 显示出对顺铂诱导的 HEI-OC1 细胞损伤的保护作用。我们还测量了 RPT 对顺铂处理细胞的脂质过氧化作用以及对超氧自由基、羟基自由基、过氧化氢和 DPPH 自由基的清除活性的影响。RPT 以剂量依赖性方式降低顺铂诱导的脂质过氧化作用。此外，RPT 对超氧自由基、羟基自由基、过氧化氢和 DPPH 自由基具有很强的清除活性。这些结果表明，RPT 通过抑制脂质过氧化作用和清除自由基的活性来保护顺铂诱导的 HEI-OC1 细胞损伤。
