Yu Hyeon-Hee, Seo Se-Jeong, Kim Yeon-Hwa, Lee Hae-Youn, Park Rae-Kil, So Hong-Seob, Jang Seon Ll, You Yong-Ouk
Department of Food and Nutrition, Kunsan National University, Kunsan, South Korea.
J Ethnopharmacol. 2006 Oct 11;107(3):383-8. doi: 10.1016/j.jep.2006.03.024. Epub 2006 Apr 6.
The steamed root of Rehmannia glutinosa has been used in traditional Oriental Medicine for treatment of inner ear diseases, such as tinnitus and hearing loss. In the present study, we showed that the ethanol extract of steamed roots of Rehmannia glutinosa (SRG) protected HEI-OC1 auditory cells from cisplatin cytotoxicity in a dose-dependent fashion. In addition, to investigate the protection mechanism of SRG on cisplatin cytotoxicity towards HEI-OC1, we measured the effects of SRG on lipid peroxidation of cisplatin treated cells as well as scavenging activities against superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. SRG (5-100 microg/ml) had protective effect against the cisplatin-induced HEI-OC1 cell damage and reduced lipid peroxidation in a dose-dependent manner. Furthermore, SRG showed strong scavenging activity against superoxide radical, hydroxyl radical, hydrogen peroxide, and DPPH radical. These results indicate that SRG protects cisplatin-induced HEI-OC1 cell damage through inhibition of lipid peroxidation and scavenging activities of free radials.
地黄蒸制后的根在传统东方医学中一直用于治疗内耳疾病，如耳鸣和听力损失。在本研究中，我们发现地黄蒸制后的根（SRG）的乙醇提取物以剂量依赖的方式保护HEI-OC1听觉细胞免受顺铂的细胞毒性作用。此外，为了研究SRG对顺铂对HEI-OC1细胞毒性的保护机制，我们测量了SRG对顺铂处理细胞脂质过氧化的影响以及对超氧自由基、羟自由基、过氧化氢和DPPH自由基的清除活性。SRG（5-100微克/毫升）对顺铂诱导的HEI-OC1细胞损伤具有保护作用，并以剂量依赖的方式减少脂质过氧化。此外，SRG对超氧自由基、羟自由基、过氧化氢和DPPH自由基表现出较强的清除活性。这些结果表明，SRG通过抑制脂质过氧化和清除自由基来保护顺铂诱导的HEI-OC1细胞损伤。
