Cheng Chun-Lin, Feng Yi, He Xiang, Lin Peng, Liang Shu-Jia, Yi Zhi-Qiang, He Jian-Mei, Hu Yuan-Yuan, Xing Hui, Fan Yan, Wu Shi-Liang, Shao Yi-Ming
National Center for AIDS/STD Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Jul;30(7):720-5.
To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in the four provinces of southern China.
Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogenetic methods and characterized by calculating the genetic distance and Entropy analysis.
Two main epidemic clusters were found to exist in the CRF01_AE strains from 210 HIV-1 CRF01_ AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster I, which including 123 samples. The strains in cluster II, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster I was obviously less than that of cluster II. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env were significantly different between the two clusters. When compared with cluster II, the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster I.
This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The virus in cluster I was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster II was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam.
分析中国南方四省流行的HIV-1 CRF01_AE毒株的基因特征。
收集2006年广东省、广西壮族自治区、江西省和湖南省新诊断的HIV-1感染者的血浆样本。使用RT和巢式PCR方法从血浆中提取的RNA模板扩增gag和env基因片段。通过系统发育方法分析CRF01_AE序列，并通过计算遗传距离和熵分析进行特征描述。
在中国南方4省收集的210例HIV-1 CRF01_AE感染者的CRF01_AE毒株中发现存在两个主要流行簇。发现没有国际参考毒株与包含123个样本的簇I密切相关。簇II中的毒株由57例样本组成，与在越南鉴定的毒株密切相关。gag和env基因的遗传距离分析表明，簇I的多样性明显低于簇II。核苷酸多态性数据显示，两个簇之间gag基因中42个位点和env基因中40个位点的核苷酸组成存在显著差异。与簇II相比，簇I中61个核苷酸位点的多态性降低，但21个位点的多态性增加。
这是首次报道描述中国南方4省流行的CRF01_AE毒株中存在两个主要流行簇。簇I中的病毒是该地区的优势毒株，传播时间较短，在HIV感染人群中所占比例较高，可能属于具有某些促进病毒传播特征的CRF01_AE毒株。簇II中的病毒与来自越南的CRF01_AE毒株高度同源，似乎在中国和越南边境地区的人群中发生过几次流行事件。
