Li Qin-zi, Kong Ling-fei, Zhang Shu-na, Zhong Zhao-shuang, Zhang Bao-hui, Liu Xiao-feng
China Medical University Institute of Respiratory Disease, First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2009 Jun;32(6):426-9.
To observe the expression of substance P (SP) in the airway mucosa of guinea pigs with repetitive esophageal stimulation by hydrochloric acid (HCL).
Twenty adult guinea pigs were randomly divided into 2 groups (n = 10 each): (1) The HCL model group: On the day of experimentation, guinea pigs were maintained under ketamine anesthesia. A 5F catheter was inserted orally into the lumen of the middle and lower esophagus. The esophagus of each animal was perfused with HCl-P for 20 min/d for 14 d. (2) The PBS control group: The esophagus of each animal was perfused with PBS instead. The bronchial responsiveness to Ach given intravenously with increasing doses (3.125, 6.25, 12.5, 25, 50, 100 microg/kg) was measured after the last perfusion. The left lung was isolated for pathological examination. Lung sections were stained with hematoxylin and eosin, and other sections were prepared for immunohistochemistry using monoclonal antibodies against SP.
In response to increasing doses of ACh, all guinea pigs showed dose-dependent increases in R(L). However, when the dose of ACh was increased to 25 microg/kg, the airway responsiveness increased significantly in the HCl-P model animals compared with the PBS control group (t values = 43.057, 51.410, 57.359 respectively, all P<0.01). The mean gray values of SP decreased significantly in the tracheal epithelia and the distal airway walls of the model group compared with the PBS control group (t values = 3.44, 2.16 respectively, all P<0.01).
There was airway neurogenic inflammation in guinea pigs with repetitive esophageal stimulation by HCL, which maybe closely related to the pathogenesis of gastro-esophageal reflux disease.
观察盐酸（HCl）反复食管刺激的豚鼠气道黏膜中P物质（SP）的表达。
将20只成年豚鼠随机分为2组（每组n = 10）：（1）HCl模型组：实验当天，豚鼠在氯胺酮麻醉下维持。将一根5F导管经口插入食管中下段管腔。每只动物的食管用HCl-P灌注20分钟/天，共14天。（2）PBS对照组：每只动物的食管改为用PBS灌注。在最后一次灌注后，测量静脉注射递增剂量（3.125、6.25、12.5、25、50、100μg/kg）乙酰胆碱（Ach）时的支气管反应性。分离左肺进行病理检查。肺组织切片用苏木精和伊红染色，其他切片用抗SP单克隆抗体进行免疫组织化学制备。
随着Ach剂量增加，所有豚鼠的R(L)均呈剂量依赖性增加。然而，当Ach剂量增加到25μg/kg时，与PBS对照组相比HCl-P模型动物的气道反应性显著增加（t值分别为43.057、51.410、57.359，均P<0.01）。与PBS对照组相比，模型组气管上皮和远端气道壁中SP的平均灰度值显著降低（t值分别为3.44、2.16，均P<0.01）。
HCl反复食管刺激的豚鼠存在气道神经源性炎症，这可能与胃食管反流病的发病机制密切相关。
