Institute of Respiratory Diseases, First Affiliated Hospital, China Medical University, Shenyang, China.
Pathobiology. 2010;77(3):163-8. doi: 10.1159/000292650. Epub 2010 May 28.
Gastroesophageal reflux disease (GERD) has been linked to a number of extra-esophageal symptoms and disorders, primarily in the respiratory tract. Current animal models of reflux esophagitis are adapted to diseases of the digestive system, rather than to reflux-associated respiratory symptoms. The aim of this study was to evaluate a novel external esophageal perfusion model to induce esophageal, tracheal and pneumonic histological injury similar to that associated with GERD.
Twenty guinea pigs were randomized to the acid-treated or PBS-treated group. Esophageal catheters were used to perfuse the esophageal lumen of guinea pigs with hydrochloric acid containing 1 g/l pepsin or PBS for 14 days. The total cell number and cell differential counts in bronchoalveolar lavage fluid (BALF) were determined 24 h after the last perfusion. Histological changes in the esophageal, tracheal and pneumonic tissues were observed by hematoxylin-eosin staining.
The numbers of lymphocytes, eosinophils and total inflammatory cells in the BALF were significantly higher in acid-perfused than PBS-perfused animals. Histological evidence suggested esophageal and pneumonic inflammations were prominent in acid-treated animals.
Repetitive, acid-perfused, esophageal events copied the animal models of reflux esophagitis, and elicited inflammatory responses in the airways and lungs of guinea pigs.
胃食管反流病（GERD）与许多食管外症状和疾病有关，主要涉及呼吸道。目前的反流性食管炎动物模型适用于消化系统疾病，而不是与反流相关的呼吸道症状。本研究旨在评估一种新的食管外灌注模型，以诱导类似于 GERD 的食管、气管和肺炎组织损伤。
将 20 只豚鼠随机分为酸处理组或 PBS 处理组。使用食管导管用含 1 g/l 胃蛋白酶的盐酸或 PBS 对豚鼠的食管腔进行灌注 14 天。最后一次灌注后 24 小时，测定支气管肺泡灌洗液（BALF）中的总细胞数和细胞分类计数。通过苏木精-伊红染色观察食管、气管和肺炎组织的组织学变化。
与 PBS 灌注组相比，酸灌注组 BALF 中的淋巴细胞、嗜酸性粒细胞和总炎症细胞数明显增加。组织学证据表明，酸处理组的食管和肺炎均有明显炎症。
重复的、酸灌注的食管事件复制了反流性食管炎的动物模型，并引起了豚鼠气道和肺部的炎症反应。
