Jiang Hua, Ma Li-Jun, Cong Hong-Liang, Jiang Nan, Mao Yong-Min, Xu Mei-Lin, Cui Rang-Zhuang
Department of Cardiology, Tianjin Chest Hospital, Tianjin Cardiology Research Institute, Tianjin 300051, China.
Zhonghua Nei Ke Za Zhi. 2009 Jul;48(7):535-8.
To examine the expression of angiotensin II (Ang II) receptor subtypes in human left and right atrial tissue in atrial fibrillation underlying rheumatic heart disease.
Atrial tissue samples were obtained from 39 patients with rheumatic heart disease, 25 with atrial fibrillation (AF) and 14 with sinus rhythm(SR) during open heart surgery. AT1 and AT2 mRNA levels were measured with semi-quantitative reverse transcription polymerase chain reaction techniques. AT1 and AT2 protein levels were measured with immunohistochemical techniques.
Compared with that of the SR group, left atrial inner diameter was significantly increased in the patients of the AF group. The AT1 mRNA and protein levels in the LA significantly increased in patients with AF compared with those in patients with SR (P < 0.05), whereas AT2 mRNA and protein were not significantly altered. Investigations of Ang II receptor subtypes' mRNA and protein levels in the RA did not exhibit any significant changes either in AT1 or AT2 in patients with AF and SR.
AF is associated with an up-regulation of AT1 in LA, but does not appear to influence the AT2 expression. This may indicate a possible pathophysiologic role for renin-angiotensin system in the development of AF. The series of effects mediated by AT1 activation may be one of the molecular mechanisms involved in the process of atrial remodeling.
研究风湿性心脏病合并心房颤动患者左右心房组织中血管紧张素II（Ang II）受体亚型的表达情况。
选取39例风湿性心脏病患者，其中25例合并心房颤动（AF），14例为窦性心律（SR），在心脏直视手术中获取心房组织样本。采用半定量逆转录聚合酶链反应技术检测AT1和AT2 mRNA水平，采用免疫组织化学技术检测AT1和AT2蛋白水平。
与SR组相比，AF组患者左心房内径显著增加。与SR患者相比，AF患者左心房中AT1 mRNA和蛋白水平显著升高（P < 0.05），而AT2 mRNA和蛋白水平无显著变化。对AF和SR患者右心房中Ang II受体亚型的mRNA和蛋白水平进行研究，结果显示AT1或AT2均未出现任何显著变化。
AF与左心房中AT1上调有关，但似乎不影响AT2的表达。这可能表明肾素-血管紧张素系统在AF发生发展过程中可能具有病理生理作用。AT1激活介导的一系列效应可能是心房重构过程中涉及的分子机制之一。
