U.S. Army Engineering Research and Development Center, Construction Engineering Research Laboratory, Champaign, Illinois 61826, USA.
Langmuir. 2010 Jan 19;26(2):613-7. doi: 10.1021/la902564n.
When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures were not observed in control reactions lacking ligase or adenosine triphosphate. Raman spectroscopy analysis revealed no spectra indicative of carbon nanotubes in ligase-unamended controls; however, spectra were observed in radial breathing mode and in the G and G' bands in reactions containing ligase. Furthermore, the addition of deoxyribonuclease to the ligated reactions dispersed the aggregates, and a reduction in Raman spectral intensity was observed.
当嘧啶功能化的碳纳米管与单链 DNA 连接酶孵育时，观察到宏观聚集体的形成。湿细胞透射电子显微镜成像显示，纳米管径向结合形成 3D 格子状结构。在缺乏连接酶或三磷酸腺苷的对照反应中没有观察到这些结构。拉曼光谱分析显示，在未经连接酶修饰的对照物中没有碳纳米管的特征谱；然而，在含有连接酶的反应中，在径向呼吸模式和 G 和 G'带中观察到了谱。此外，将脱氧核糖核酸酶添加到连接的反应中会分散聚集体，并观察到拉曼光谱强度降低。
