Korean Bioinformation Center, KRIBB, Daejeon, 305-806, Korea.
BMC Genomics. 2009 Dec 3;10 Suppl 3(Suppl 3):S20. doi: 10.1186/1471-2164-10-S3-S20.
Mitochondria play a vital role in the energy production and apoptotic process of eukaryotic cells. Proteins in the mitochondria are encoded by nuclear and mitochondrial genes. Owing to a large increase in the number of identified mitochondrial protein sequences and completed mitochondrial genomes, it has become necessary to provide a web-based database of mitochondrial protein information.
We present 'MitoInteractome', a consolidated web-based portal containing a wealth of information on predicted protein-protein interactions, physico-chemical properties, polymorphism, and diseases related to the mitochondrial proteome. MitoInteractome contains 6,549 protein sequences which were extracted from the following databases: SwissProt, MitoP, MitoProteome, HPRD and Gene Ontology database. The first general mitochondrial interactome has been constructed based on the concept of 'homologous interaction' using PSIMAP (Protein Structural Interactome MAP) and PEIMAP (Protein Experimental Interactome MAP). Using the above mentioned methods, protein-protein interactions were predicted for 74 species. The mitochondrial protein interaction data of humans was used to construct a network for the aging process. Analysis of the 'aging network' gave us vital insights into the interactions among proteins that influence the aging process.
MitoInteractome is a comprehensive database that would (1) aid in increasing our understanding of the molecular functions and interaction networks of mitochondrial proteins, (2) help in identifying new target proteins for experimental research using predicted protein-protein interaction information, and (3) help in identifying biomarkers for diagnosis and new molecular targets for drug development related to mitochondria. MitoInteractome is available at http://mitointeractome.kobic.kr/.
线粒体在真核细胞的能量产生和凋亡过程中起着至关重要的作用。线粒体中的蛋白质由核基因和线粒体基因编码。由于鉴定出的线粒体蛋白序列和完成的线粒体基因组数量的大量增加，因此有必要提供一个基于网络的线粒体蛋白信息数据库。
我们提出了“MitoInteractome”，这是一个基于网络的综合门户，包含了大量关于预测蛋白-蛋白相互作用、理化性质、多态性以及与线粒体蛋白质组相关的疾病的信息。MitoInteractome 包含 6549 个从以下数据库中提取的蛋白质序列：SwissProt、MitoP、MitoProteome、HPRD 和 Gene Ontology 数据库。基于“同源相互作用”的概念，首次构建了一般的线粒体相互作用组，使用 PSIMAP（蛋白质结构相互作用图）和 PEIMAP（蛋白质实验相互作用图）。使用上述方法，预测了 74 个物种的蛋白-蛋白相互作用。利用上述方法，我们利用人类的线粒体蛋白相互作用数据构建了一个衰老过程的网络。对“衰老网络”的分析使我们深入了解了影响衰老过程的蛋白质之间的相互作用。
MitoInteractome 是一个综合性的数据库，将有助于：（1）提高我们对线粒体蛋白的分子功能和相互作用网络的理解；（2）利用预测的蛋白-蛋白相互作用信息，帮助识别新的实验研究目标蛋白；（3）有助于鉴定与线粒体相关的诊断生物标志物和新的药物开发分子靶标。MitoInteractome 可在 http://mitointeractome.kobic.kr/ 获得。
