Laboratoire de Biosurveillance de l'Environnement, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia.
Mar Environ Res. 2010 May;69(4):248-53. doi: 10.1016/j.marenvres.2009.10.018. Epub 2009 Nov 10.
The effects of two lubricating oils on nematode assemblages of a Tunisian lagoon were investigated in a microcosm experiment. Sediment from a pristine site in Ghar El Melh lagoon (Western Mediterranean) was treated with either mineral oil (Mobil 20 W-50), a synthetic lubricant (Mobil 0 W-40), the same two lubricants after use in a vehicle, and effects were examined after 5 weeks. Univariate analysis showed significant differences between most univariate indices of the nematode assemblages in all the lubricant treatments as compared to the control. Total nematode abundance (I), species richness (d) and number of species (S) decreased significantly in all lubricant contaminated microcosms. However, evenness was not affected in all treated replicates except in used mineral lubricant treatment where it was significantly higher than in the control. Diversity (H') was only altered in synthetic lubricant treatments. Results from multivariate analyses of the species abundance data demonstrated that responses of nematode species to the two lubricants treatments were varied: Daptonema trabeculosum was eliminated in all lubricant treatments and seemed to be an intolerant species to oil contamination. Spirinia gerlachi increased in mineral lubricant treatments ("clean" and used) but was eliminated in all synthetic lubricant treatments. This species could be categorized as "resistant" to mineral oil contamination and intolerant to synthetic lubricant contamination. Terschellingia longicaudata increased only in synthetic lubricant treatments ("clean" and used) and appeared to be a "synthetic oil-resistant" species.
在一项微宇宙实验中，研究了两种润滑油对突尼斯泻湖线虫组合的影响。从盖勒梅尔泻湖（西地中海）原始地点采集的沉积物用矿物油（美孚 20W-50）、合成润滑剂（美孚 0W-40）处理，或用车辆使用后的相同两种润滑剂处理，并在 5 周后检查效果。单变量分析表明，与对照相比，所有润滑油处理的线虫组合的大多数单变量指数之间存在显著差异。在所有受污染的微宇宙中，总线虫丰度（I）、物种丰富度（d）和物种数（S）均显著下降。然而，除了在使用矿物润滑剂处理中，均匀度不受所有处理重复的影响，而在对照中均匀度显著更高。多样性（H'）仅在合成润滑剂处理中发生改变。对物种丰度数据进行的多元分析结果表明，线虫物种对两种润滑油处理的反应是不同的：Daptonema trabeculosum 在所有润滑油处理中都被消除，似乎是一种对油污染不耐受的物种。Spirinia gerlachi 在矿物润滑剂处理（“清洁”和使用）中增加，但在所有合成润滑剂处理中都被消除。这种物种可以归类为对矿物油污染的“抗性”，对合成润滑剂污染的不耐受。Terschellingia longicaudata 仅在合成润滑剂处理（“清洁”和使用）中增加，似乎是一种“合成油抗性”物种。
