Institute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12, A-8010 Graz, Austria.
J Hazard Mater. 2010 Apr 15;176(1-3):125-30. doi: 10.1016/j.jhazmat.2009.11.006. Epub 2009 Nov 10.
Bacteria capable of degrading ethylene glycol dinitrate (EGDN) were isolated under aerobic and, carbon and nitrogen-limiting conditions from EGDN contaminated soil and rivers. EGDN degradation was monitored using HPLC and UV-Vis spectrometer. Among the isolated strains Bacillus subtilis GN was the best, completely degrading 6.6 mM EGDN with the concomitant release of nitrite and EGMN within 72 h. Furthermore, the level of toxicity of EGDN as measured by the bioluminescent bacteria, Vibrio fischeri was reduced by 80% when 100% of the 6.6 mM EGDN was degraded. An environmentally friendly "biodegradable explosives", was achieved by adsorbing B. subtilis GN spores onto the wood flour, an ingredient of the explosive. The incorporation of B. subtilis GN spores into the explosive formulation did not affect the quality of the explosive as confirmed by the almost unchanged detonation velocity (3410 m s(-1) compared to 3500 m s(-1) of the control), autoignition temperature, Abel test, shock and friction sensitivity test. It was also possible to achieve rapid degradation of the residues after detonation upon exposure to air and moisture.
从 EGDN 污染的土壤和河流中，在有氧和碳、氮限制条件下分离出能够降解乙二醇二硝酸酯 (EGDN) 的细菌。使用 HPLC 和紫外-可见分光光度计监测 EGDN 的降解情况。在所分离的菌株中，枯草芽孢杆菌 GN 是最好的，在 72 小时内完全降解 6.6 mM 的 EGDN，同时释放出亚硝酸盐和 EGMN。此外，当 6.6 mM 的 EGDN 完全降解时，发光细菌发光杆菌的 EGDN 毒性水平降低了 80%。通过将枯草芽孢杆菌 GN 孢子吸附到木粉上，实现了一种环保的“可生物降解的炸药”，木粉是炸药的一种成分。将枯草芽孢杆菌 GN 孢子掺入炸药配方中不会影响炸药的质量，这一点通过几乎不变的爆速（与对照相比为 3410 m s(-1)，为 3500 m s(-1)）、自燃温度、Abel 试验、冲击和摩擦敏感度试验得到了证实。在暴露于空气和湿气后，爆炸后的残留物也可以实现快速降解。
